A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors
A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors
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DOI：
10.1523/jneurosci.0254-07.2007
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发表时间：
2007-06-27
影响因子：
5.3
通讯作者：
Alvarez-Buylla, Arturo
中科院分区：
文献类型：
--
作者：
Kohwi, Minoree;Petryniak, Magdalena A.;Alvarez-Buylla, Arturo
出生后大脑的室下区（SVZ）不断产生嗅球（OB）中间神经元。我们发现钙视网膜蛋白 (+)、钙结合蛋白 (+) 和多巴胺能 (TH (+)) 球周 OB 中间神经元对应于 GABA 能细胞的不同亚型；所有这些都是在出生后的小鼠大脑中产生的，但它们的成熟和消除速度不同。胚胎外侧神经节隆起（LGE）被认为是出生后 SVZ 神经祖细胞的起源部位。一致地，胚胎 LGE 移植到成人大脑 SVZ 中产生了许多 OB 中间神经元，包括 TH (+) 和钙结合蛋白 (+) 肾小球周围中间神经元。然而，这些 LGE 移植物并未产生钙视网膜素 (+) 细胞。令人惊讶的是，移植到成人大脑 SVZ 中的大脑皮层和隔膜胚胎祖细胞也导致了 OB 中间神经元的产生，包括钙视网膜蛋白 (+) 细胞。 Dlx2 (+) OB 中间神经元的一个子集源自表达 Emx1 的细胞，Emx1 是一种在发育过程中主要局限于大脑皮层的转录因子。 Emx1 谱系衍生细胞贡献了 OB 中 GABA 能细胞的很大一部分，包括钙视网膜蛋白 (+) 中间神经元。这与皮质相反，皮质中 Emx1 谱系衍生的细胞不会分化为 GABA 能神经元。我们的结果表明，一些 OB 中间神经元源自 LGE 之外的祖细胞，并且表达传统上被认为是大脑皮层转录因子的前体细胞产生 GABA 能中间神经元。
The subventricular zone (SVZ) of the postnatal brain continuously generates olfactory bulb (OB) interneurons. We show that calretinin (+), calbindin (+), and dopaminergic (TH (+)) periglomerular OB interneurons correspond to distinct subtypes of GABAergic cells; all were produced in the postnatal mouse brain, but they matured and were eliminated at different rates. The embryonic lateral ganglionic eminence (LGE) is thought to be the site of origin of postnatal SVZ neural progenitors. Consistently, grafts of the embryonic LGE into the adult brain SVZ generated many OB interneurons, including TH (+) and calbindin (+) periglomerular interneurons. However, calretinin (+) cells were not produced from these LGE grafts. Surprisingly, pallial and septal embryonic progenitors transplanted into the adult brain SVZ also resulted in the generation of OB interneurons, including calretinin (+) cells. A subset of Dlx2 (+) OB interneurons was derived from cells expressing Emx1, a transcription factor largely restricted to the pallium during development. Emx1 lineage-derived cells contributed a substantial portion of GABAergic cells in the OB, including calretinin (+) interneurons. This is in contrast to cortex, in which Emx1 lineage- derived cells do not differentiate into GABAergic neurons. Our results suggest that some OB interneurons are derived from progenitors outside the LGE and that precursors expressing what has classically been considered a pallial transcription factor generate GABAergic interneurons.