The use of reverse transcription-polymerase chain reaction (RT-PCR) for monitoring aflatoxin production in Aspergillus parasiticus 439
The use of reverse transcription-polymerase chain reaction (RT-PCR) for monitoring aflatoxin production in Aspergillus parasiticus 439
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DOI：
10.1016/s0168-1605(00)00277-4
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发表时间：
2000-05-25
影响因子：
5.4
通讯作者：
Dobson, ADW
中科院分区：
文献类型：
--
作者：
Sweeney, MJ;Pàmies, P;Dobson, ADW
建立了一种基于反转录PCR（RT-PCR）的检测方法，用于检测黄曲霉毒素基因在帕瓦西曲霉中的表达。产黄曲霉毒素A.用从黄曲霉毒素生物合成途径的两个基因设计的特异性引物，通过RT-PCR扩增并随时间监测在黄曲霉毒素允许和非允许培养基中生长的寄生菌439。通过监测管家β-微管蛋白基因来评估两种培养基中的基因转录，并通过薄层色谱法将黄曲霉毒素产生与转录相关联。这种RT-PCR技术有可能被用作一种工具，以调查各种生理因素对黄曲霉毒素基因的转录的影响。(C)2000 Elsevier Science B. V.保留所有权利。
A detection system based on reverse transcription PCR (RT-PCR) has been developed to monitor aflatoxin gene expression in Aspergillus pavasiticus. Total RNAs of aflatoxigenic A. parasiticus 439 grown in aflatoxin permissive and non-permissive media were amplified and monitored over time by RT-PCR with specific primers designed from two genes of the aflatoxin biosynthetic pathway. Gene transcription in both media was assessed by monitoring the house keeping beta-tubulin gene and aflatoxin production was correlated with transcription by thin layer chromatography. This RT-PCR technique has the potential to be employed as a tool to investigate the effects of a variety of physiological factors on the transcription of the aflatoxin genes. (C) 2000 Elsevier Science B.V. All rights reserved.