Crop milk protein is synthesised following activation of the IRS1/Akt/TOR signalling pathway in the domestic pigeon (Columba livia)
Crop milk protein is synthesised following activation of the IRS1/Akt/TOR signalling pathway in the domestic pigeon (Columba livia)
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家鸽 (Columba livia) 的 IRS1/Akt/TOR 信号通路激活后合成作物乳蛋白
DOI：
10.1080/00071668.2016.1219694
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发表时间：
2016-12-01
影响因子：
2
通讯作者：
Wang, X. -Q.
中科院分区：
文献类型：
--
作者：
Hu, X. -C.;Gao, C. -Q;Wang, X. -Q.
1.为研究胰岛素受体物质1（IRS 1）/蛋白激酶B（Akt）/雷帕霉素靶蛋白（TOR）信号通路的激活对家鸽乳蛋白合成的影响，采集10只1日龄乳鸽乳，分析乳蛋白的营养成分。在非繁殖期和泌乳期第1天分别采集5对种鸽的血样，测定血清催乳素和胰岛素水平，在孵化期第14、16天和泌乳期第1、3、7天分别采集5对种鸽的作物样品。对作物样品进行了评估，以确定作物重量和厚度的变化以及IRS 1/Akt/TOR信号通路相关蛋白表达模式的变化。结果表明，催乳素诱导作物相对重量和厚度逐渐增加，乳鸽乳中粗蛋白含量为64.1%，粗蛋白含量为29.7%，泌乳期血清催乳素和胰岛素水平显著高于非繁殖期。与非繁殖鸽相比，在哺乳期鸽的生殖器中，磷酸化IRS 1、磷酸化Akt、磷酸化TOR、磷酸化核糖体蛋白S6激酶、磷酸化S6、磷酸化真核起始因子4 E结合蛋白1和真核起始因子4 E的表达显著上调。催乳素可能引起作物组织的变化，形成作物乳合成的生理结构。此外，作物乳蛋白的合成通过IRS 1/Akt/TOR信号通路的激活来调节。
1. The experiment was conducted to study whether insulin receptor substance 1 (IRS1) / Protein kinase B (Akt)/target of the rapamycin (TOR) signalling pathway activation stimulates crop milk protein synthesis in the domestic pigeon (Columba livia).Crop milk was collected from ten 1-d-old squabs and analysed for nutrient content. During the non-breeding period and the first day of lactation, blood samples were collected from 5 pairs of breeding pigeons and the levels of prolactin and insulin were determined.Crop samples were collected from 5 pairs of breeders at d 14 and 16 of the incubation period and d 1, 3 and 7 of the lactation period. Crop samples were evaluated for changes in crop weight and thickness and changes in the expression patterns of IRS1/Akt/TOR signalling pathway-related proteins.The results demonstrated that prolactin induces a gradual increase in the relative weight and thickness of the crop, with crops reaching a maximum size at the third day of lactation.Pigeon crop milk contains 64.1% crude protein and 29.7% crude fat based on dry weight.Serum prolactin and insulin levels in the lactation period were significantly higher than those in the non-breeding period. Compared with non-breeding pigeons, the expression of the phosphorylated IRS1 phosphorylated Akt, phosphorylated TOR, phosphorylated ribosomal protein S6 kinase, phosphorylated S6, phosphorylated eukaryotic initiation factor 4E binding protein 1 and eukaryotic initiation factor 4E were significantly up-regulated in the crop of pigeons in the lactation period.In conclusion, prolactin might induce changes in crop tissue and form the physiological structure for crop milk synthesis. Furthermore, the synthesis of crop milk protein is regulated by activation of the IRS1/Akt/TOR signalling pathway.