Focused Ultrasound Promotes the Delivery of Gastrodin and Enhances the Protective Effect on Dopaminergic Neurons in a Mouse Model of Parkinson's Disease.
Focused Ultrasound Promotes the Delivery of Gastrodin and Enhances the Protective Effect on Dopaminergic Neurons in a Mouse Model of Parkinson's Disease.
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DOI：
10.3389/fncel.2022.884788
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发表时间：
2022
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
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作者：
Parkinson’s disease (PD) is the second most common chronic neurodegenerative disease globally; however, it lacks effective treatment at present. Focused ultrasound (FUS) combined with microbubbles could increase the efficacy of drug delivery to specific brain regions and is becoming a promising technology for the treatment of central nervous system diseases. In this study, we explored the therapeutic potential of FUS-mediated blood–brain barrier (BBB) opening of the left striatum to deliver gastrodin (GAS) in a subacute PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The concentration of GAS in the left hemisphere was detected by ultra-high performance liquid chromatography electrospray Q-Orbitrap mass spectrometry (UHPLC/ESI Q-Orbitrap) and the distribution of tyrosine hydroxylase (TH) neurons was detected by immunohistochemical staining. The expression of TH, Dopamine transporter (DAT), cleaved-caspase-3, B-cell lymphoma 2 (Bcl-2), brain-derived neurotrophic factor (BDNF), postsynaptic density protein 95 (PSD-95), and synaptophysin (SYN) protein were detected by western blotting. Analysis showed that the concentration of GAS in the left hemisphere of PD mice increased by approximately 1.8-fold after the BBB was opened. FUS-mediated GAS delivery provided optimal neuroprotective effects and was superior to the GAS or FUS control group. In addition, FUS enhanced GAS delivery significantly increased the expression of Bcl-2, BDNF, PSD-95, and SYN protein in the left striatum (P < 0.05) and reduced the levels of cleaved-caspase-3 remarkably (P = 0.001). In conclusion, the enhanced delivery by FUS effectively strengthened the protective effect of GAS on dopaminergic neurons which may be related to the reinforcement of the anti-apoptotic activity and the expression of synaptic-related proteins in the striatum. Data suggests that FUS-enhanced GAS delivery may represent a new strategy for PD treatment.
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DOI：
10.1155/2013/514095
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Kumar H;Kim IS;More SV;Kim BW;Bahk YY;Choi DK
通讯作者：
Choi DK
影响因子：
10.8
作者：
Mead BP;Kim N;Miller GW;Hodges D;Mastorakos P;Klibanov AL;Mandell JW;Hirsh J;Suk JS;Hanes J;Price RJ
通讯作者：
Price RJ
研究了纳米颗粒的最佳尺寸，以通过聚焦超声引起的血脑屏障开口辅助进入大脑。
影响因子：
4.6
作者：
Ohta S;Kikuchi E;Ishijima A;Azuma T;Sakuma I;Ito T
通讯作者：
Ito T
影响因子：
4.6
作者：
Koppula S;Alluri R;Kopalli SR
通讯作者：
Kopalli SR
影响因子：
10.8
作者：
Lin, Chung-Yin;Hsieh, Han-Yi;Liu, Hao-Li
通讯作者：
Liu, Hao-Li