Bioinspired Reactive Interfaces Based on Layered Double Hydroxides-Zn Rich Hydroxyapatite with Antibacterial Activity
Bioinspired Reactive Interfaces Based on Layered Double Hydroxides-Zn Rich Hydroxyapatite with Antibacterial Activity
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DOI：
10.1021/acsbiomaterials.0c01643
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发表时间：
2021-03-19
影响因子：
5.8
通讯作者：
Nocchetti, Morena
中科院分区：
文献类型：
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作者：
Donnadio, Anna;Bini, Marzia;Nocchetti, Morena
本工作的重点是通过结合层状双氢氧化物（LDHs）和羟基磷灰石（HA）获得的潜在生物相容性反应界面的制备和多技术表征。选择具有抗菌和骨诱导作用的金属离子Zn ~（2+）和Ga ~（3+）作为LDH的层内组分，得到ZnAl和ZnAlGa体系。这些LDH，与磷酸二氢根阴离子交换，促进HA在LDH表面的沉淀产生HA@LDH复合材料。通过Rietveld精修法进行X射线衍射定量分析，结合元素分析和显微拉曼光谱，表明形成了Ca-Zn HA混合相。扫描电子显微镜显示，在LDH的存在下，HA优先沿其a轴生长，因此主要以片状晶体的形式结晶。沿着a轴生长。LDH和HA@LDH复合物在对人成骨细胞（hFob 1.19）无细胞毒性的浓度下显示出对金黄色葡萄球菌和铜绿假单胞菌的抗菌活性，特别是当Ga阳离子存在于LDH结构中时。复合材料中HA的存在对骨结合能力和对人成骨细胞增殖的影响也进行了研究。HA能降低LDH对人成骨细胞的毒性，但不影响其骨结合能力。这项多学科研究提供了新型LDH和HA@LDH复合材料的生物化学和结构表征，还评估了其生物活性，以潜在适用于钛基假体。
This work is focused on the preparation and multi-technique characterization of potentially biocompatible reactive interfaces obtained by combining layered double hydroxides (LDHs) and hydroxyapatite (HA). Antimicrobial and osteoinductive metallic ions as Zn2+ and Ga3+ were chosen as intralayer constituents of LDH to obtain ZnAl and ZnAlGa systems. These LDHs, exchanged with dihydrogenphosphate anions, promoted the precipitation of HA on the LDH surface yielding HA@LDH composites. X-ray diffraction quantitative analysis, through the Rietveld refinement method, coupled with elemental analysis and micro-Raman spectroscopy showed the formation of a mixed Ca-Zn HA phase. Scanning electron microscopy revealed that HA, in the presence of LDH, grew preferentially along its a-axis, thus crystallizing mainly in the form of flake crystals. LDH and HA@LDH composites showed antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa at not cytotoxic concentrations for human osteoblasts (hFob 1.19), especially when Ga cations were present in the LDH structure. The effect of the presence of HA in the composites on the bone-bonding ability and on human osteoblast proliferation was also investigated. The HA seemed to reduce the toxicity of the LDH toward human osteoblast while did not affect the bone-bonding ability. This multidisciplinary study provides the bio-chemical, structural characterization of new LDH and HA@LDH composites, evaluating also their bioactivity to be potentially applicable to titanium-based prostheses.