Water-soluble dietary fibers enhance bioavailability of quercetin and a fiber derived from soybean is most effective after long-term feeding in rats.
Water-soluble dietary fibers enhance bioavailability of quercetin and a fiber derived from soybean is most effective after long-term feeding in rats.
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水溶性膳食纤维可增强槲皮素的生物利用度，而源自大豆的纤维在长期喂养大鼠后最有效。
DOI：
10.1007/s00394-019-01992-9
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Hara H.
中科院分区：
文献类型：
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作者：
Trakooncharoenvit A;Tanaka S;Mizuta E;Hira T;Hara H.
目的探讨水溶性膳食纤维（果胶、大豆纤维、瓜尔胶）对槲皮素-3-O-葡萄糖苷（Q3G，31.8%）及其葡萄糖加合物组成的槲皮素葡萄糖苷混合物（Q3GM）生物利用度的影响。方法雄性Wistar/ST大鼠饲喂含0.7%Q3GM的试验饲料，添加或不添加每种膳食纤维各5%，进行实验。 8 周。采用液相色谱串联质谱法 (LC-MS/MS) 评估第 2、4、6 和 8 周血浆、尿液和粪便样本中酶解后的总槲皮素衍生物。还测量了粪便中排泄的槲皮素葡萄糖醛酸苷。结果纤维喂养增加了盲肠重量并降低了盲肠 pH，表明盲肠发酵得到促进。血浆和尿槲皮素水平的变化揭示了槲皮素代谢的三个阶段，包括累积期、短暂期和稳定期。第 2 周，三个纤维喂养组的血浆样本中总槲皮素衍生物含量高于对照组；然而，纤维喂养组的尿排泄量在第 4 周有所增加。大豆纤维在第 6 周和第 8 周上调了血浆和尿槲皮素水平。根据苷元摄入量与尿液和粪便排泄总和之间的差异计算，细菌对槲皮素的肠道降解在补充膳食纤维后受到抑制，尤其是果胶纤维，这可能部分有助于提高槲皮素生物利用度。大豆纤维喂养的大鼠粪便中槲皮素葡萄糖醛酸排泄量较高，表明结肠中的β-葡萄糖醛酸酶减少。结论水溶性膳食纤维，尤其是大豆纤维，可增强长期喂养后槲皮素的生物利用度，并可能促进槲皮素的预防疾病作用。
PurposeTo investigate the effects of water-soluble dietary fibers (pectin, soybean fiber, and guar gum) on the bioavailability of quercetin glucoside mixture (Q3GM) comprising quercetin-3-O-glucoside (Q3G, 31.8%) and its glucose adducts.MethodsMale Wistar/ST rats were fed test diet containing 0.7% Q3GM with or without 5% of each dietary fiber for 8 weeks. Total quercetin derivatives were evaluated with liquid chromatograph tandem mass spectrometry (LC–MS/MS) as total quercetin derivatives after enzymatic deconjugation in plasma, urine, and fecal samples on week 2, 4, 6 and 8. Quercetin glucuronides excreted in feces were also measured.ResultsFiber feeding elevated cecal weight and reduced cecal pH, indicative of cecal fermentation promotion. Changes in plasma and urinary quercetin levels revealed three phases of quercetin metabolism, including cumulative, transient, and stable phases. On week 2, total quercetin derivatives were higher in plasma samples from three fiber-fed groups than those control groups; however, urinary excretion increased in fiber-fed groups on week 4. Soybean fiber upregulated plasma and urinary quercetin levels on week 6 and 8. Intestinal degradation of quercetin by bacteria, calculated from differences between aglycone ingestion and sum of urinary and fecal excretion, was suppressed after dietary fiber supplementation especially in pectin fiber, which may partly contribute to the increase in quercetin bioavailability. Fecal quercetin glucuronide excretion was high in soybean fiber-fed rats, suggestive of the reduction of β-glucuronidase in colon.ConclusionWater-soluble dietary fibers, especially soybean fiber, enhanced quercetin bioavailability after chronic feeding and may promote beneficial effects of quercetin on disease prevention.