ECHINOCOCCUS-MULTILOCULARIS - PARASITE-SPECIFIC HUMORAL AND CELLULAR IMMUNE-RESPONSE SUBSETS IN MOUSE STRAINS SUSCEPTIBLE (AKR, C57B1/6J) OR RESISTANT (C57B1/10) TO SECONDARY ALVEOLAR ECHINOCOCCOSIS
ECHINOCOCCUS-MULTILOCULARIS - PARASITE-SPECIFIC HUMORAL AND CELLULAR IMMUNE-RESPONSE SUBSETS IN MOUSE STRAINS SUSCEPTIBLE (AKR, C57B1/6J) OR RESISTANT (C57B1/10) TO SECONDARY ALVEOLAR ECHINOCOCCOSIS
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DOI：
10.1111/j.1365-2249.1994.tb06549.x
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发表时间：
1994-05-01
影响因子：
4.6
通讯作者：
TANNER, I
中科院分区：
文献类型：
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作者：
GOTTSTEIN, B;WUNDERLIN, E;TANNER, I
对继发性泡型包虫病(感染多房棘球绦虫)的高敏(AKR和C57B1/6J)和相对耐药(C57B1/10)小鼠进行了寄生虫特异性体液免疫和细胞免疫应答的研究。在所有三个品系的小鼠中，对体外抗原刺激的淋巴结细胞的寄生虫特异性增殖性免疫反应仍然很弱。到第30天，在所有三个品系的小鼠中，CD4(+)淋巴母细胞占原始细胞总数的主导地位。然而，CD8(+)原始细胞的比例出人意料地高；到第90天，易感(AKR和C57B1/6J)小鼠的CD8(+)细胞比例显著增加，而耐药(C57B1/10)小鼠则没有。敏感但不耐药的小鼠在第90天表现出淋巴细胞对刀豆蛋白A(ConA)刺激的反应性显著降低。用ELISA法分析C57B1/10小鼠的体液免疫应答表明，C57B1/10小鼠的抵抗力与宿主合成抗IgG3和IgG1同型Em2抗体的能力有关。Em2是一种板层凝集素结合的碳水化合物抗原。在易感AKR和C57B1/6J小鼠中，检测到低水平的IgG2a同型抗Em2抗体。然而，没有IgG3/IgG1同型的抗Em2抗体。免疫印迹分析证实了亚类特异性免疫球蛋白反应的差异。我们的发现表明，抗原识别的差异(相对于体液和细胞免疫成分的亚群)可能由非H-2基因(S)控制，再加上CD8(+)细胞和/或相应细胞因子调节的免疫抑制，可能决定了实验性多房埃希氏菌感染的易感性或耐药性。
Parasite-specific humoral and cell-mediated immune responses were investigated in highly susceptible (AKR and C57B1/6J) and relatively resistant (C57B1/10) mice undergoing secondary alveolar echinococcosis (infection with Echinococcus multilocularis metacestode). The parasite-specific proliferative immune response of lymph node cells upon in vitro antigen stimulation remained weak in all three mouse strains. By day 30 p.i., CD4(+) lymphoblast cells dominated the total population of blast cells in all three mouse strains. There was, however, an unexpectedly high proportion of CD8(+) blast cells; by day 90 p.i., a marked proportional increase in CD8(+) cells was seen in susceptible (AKR and C57B1/6J), but not in resistant (C57B1/10) mice. Susceptible, but not resistant mice exhibited a significantly decreased responsiveness of lymph node cells to concanavalin A (Con A) stimulation on day 90 p.i. Analysis of the humoral immune response by ELISA showed that resistance in C57B1/10 mice was associated with the ability of the host to synthesize antibodies to Em2 of the IgG3 and IgG1 isotype. Em2 is a lectin-binding carbohydrate antigen of the laminated layer. In susceptible AKR and C57B1/6J mice, low levels of anti-Em2 antibodies of the IgG2a isotype were detected. Anti-Em2 antibodies of the IgG3/IgG1 isotype, however, were absent. Differences in subclass-specific IgG responses were confirmed by immunoblot analyses. Our findings suggest that differences in antigen recognition (with respect to subsets of humoral and cellular immune components), probably controlled by non-H-2 gene(s), coupled to immune suppression modulated by CD8(+) cells and/or respective cytokines, may determine susceptibility or resistance in experimental infection with E. multilocularis.