PMR6, a pectate lyase-like gene required for powdery mildew susceptibility in Arabidopsis
PMR6, a pectate lyase-like gene required for powdery mildew susceptibility in Arabidopsis
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DOI：
10.1105/tpc.003509
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发表时间：
2002-09-01
期刊：
影响因子：
11.6
通讯作者：
Somerville, SC
中科院分区：
文献类型：
--
作者：
Vogel, JP;Raab, TK;Somerville, SC
植物病原体生长和繁殖所需的植物基因在很大程度上是未知的。为了鉴定这些基因，我们分离了不支持白粉病病原体白粉病正常生长的拟南芥突变体。在这里，我们报告了这些基因之一 PMR6 的克隆和表征。 PMR6 编码具有新型 C 末端结构域的果胶酸裂解酶样蛋白。傅里叶变换红外光谱显示，与其预测的基因功能一致，PMR6 的突变改变了植物细胞壁的组成。 pmr6介导的抗性既不需要水杨酸，也不需要感知茉莉酸或乙烯的能力，表明抗性机制不需要激活已明确描述的防御途径。因此，pmr6抗性代表了一种新形式的疾病抗性，其基于相容相互作用期间所需基因的丢失，而不是已知宿主防御途径的激活。
The plant genes required for the growth and reproduction of plant pathogens are largely unknown. In an effort to identify these genes, we isolated Arabidopsis mutants that do not support the normal growth of the powdery mildew pathogen Erysiphe cichoracearum. Here, we report on the cloning and characterization of one of these genes, PMR6. PMR6 encodes a pectate lyase-like protein with a novel C-terminal domain. Consistent with its predicted gene function, mutations in PMR6 alter the composition of the plant cell wall, as shown by Fourier transform infrared spectroscopy. pmr6-mediated resistance requires neither salicylic acid nor the ability to perceive jasmonic acid or ethylene, indicating that the resistance mechanism does not require the activation of well-described defense pathways. Thus, pmr6 resistance represents a novel form of disease resistance based on the loss of a gene required during a compatible interaction rather than the activation of known host defense pathways.