Cardiac myocytes and dendritic cells harbor human immunodeficiency virus in infected patients with and without cardiac dysfunction: detection by multiplex, nested, polymerase chain reaction in individually microdissected cells from right ventricular endom
Cardiac myocytes and dendritic cells harbor human immunodeficiency virus in infected patients with and without cardiac dysfunction: detection by multiplex, nested, polymerase chain reaction in individually microdissected cells from right ventricular endom
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在患有或不患有心脏功能障碍的感染患者中，心肌细胞和树突状细胞携带人类免疫缺陷病毒：通过多重巢式聚合酶链反应对来自右心室子宫内膜的单个微解剖细胞进行检测
DOI：
10.1016/0002-9149(91)90288-v
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Garrett,CT
中科院分区：
文献类型：
--
作者：
Rodriguez,ER;Nasim,S;Hsia,J;Sandin,RL;Ferreira,A;Hilliard,BA;Ross,AM;Garrett,CT
215名感染人类免疫缺陷病毒（HIV）的患者参加了一项HIV相关心脏病的前瞻性纵向研究。评估包括信号平均心电图和超声心动图。15例患者行心肌膜活检，5例有心血管症状，10例无。心肌细胞或树突状细胞通过单个细胞显微解剖制备，从其他细胞类型（如间质细胞或循环血液成分）中进行分类。通过多重巢式聚合酶链反应，在每种类型的15 - 20个细胞样本中扩增出HIV前病毒序列，并与HIV-1 gag和pol基因内特定区域的32p标记探针杂交。结果显示，5例有心脏症状的患者中有2例心肌细胞中存在HIV序列，10例无心脏症状的患者中有6例心肌细胞中存在HIV序列。因此，症状性HIV心肌病似乎不是病毒对心肌细胞的直接影响。在树突状细胞中，5例出现心脏症状的患者中有5例检测到HIV序列，10例心室功能明显正常的患者中有8例检测到HIV序列。此外，有症状的患者心肌中的树突状细胞比无症状的患者多。我们的研究是第一个直接检测纯化心肌细胞中HIV基因组的研究，这些心肌细胞来自有或没有心功能障碍的患者。我们的发现不支持病毒在心肌功能障碍中的直接作用。然而，这些结果确实表明，间质树突状细胞可能以某种方式参与了hiv感染患者心功能障碍的发展。
Two hundred fifteen patients infected with human immunodeficiency viras (HIV) participated in a prospective longitudinal study of HIV-related heart disease. Evaluation included signal-averaged etectrocardiography and echocardiography. Fifteen patients underwent endomyocardial biopsy, 5 had cardiovascular symptoms and 10 did not. Cardiac myocytes or dendritic cells were prepared by individual cell microdissection to sort them from other cell types such as interstitial cells or circulating blood elements. HIV proviral sequences were amplified in samples of 15 to 20 cells of each type by multiplex, nested, polymerase chain reaction and hybridized to32P-labeled probes specific for regions within the gag and pol genes of HIV-1. The results showed the presence of HIV sequences in myocytes of 2 of 5 patients with cardiac symptoms and in 6 of 10 without. Thus, symptomatic HIV cardiomyopathy did not appear to be a direct consequence of the viras on myocardial cells. In dendritic cells, HIV sequences were detected in 5 of 5 patients with cardiac symptoms and in 8 of 10 with apparently normal ventricular function. Furthermore, dendritic cells were somewhat more numerous in the myocardium of symptomatic than asymptomatic patients. Our studies are the first to directly detect the HIV genome in purified cardiac myocytes from patients with and without cardiac dysfunction. Our findings do not support a direct role of the viras in myocardial dysfunction. However, the results do suggest that the interstitial dendritic cells may be involved in some manner in the development of cardiac dysfunction observed in HIV-infected patients.