1ST OCCURRENCE OF TETRODOTOXIN IN A DENDROBATID FROG (COLOSTETHUS-INGUINALIS), WITH FURTHER REPORTS FOR THE BUFONID GENUS ATELOPUS
1ST OCCURRENCE OF TETRODOTOXIN IN A DENDROBATID FROG (COLOSTETHUS-INGUINALIS), WITH FURTHER REPORTS FOR THE BUFONID GENUS ATELOPUS
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DOI：
10.1016/0041-0101(94)90081-7
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发表时间：
1994-03-01
期刊：
影响因子：
2.8
通讯作者：
YASUMOTO, T
中科院分区：
文献类型：
--
作者：
DALY, JW;GUSOVSKY, F;YASUMOTO, T
用荧光高效液相色谱法对采自松毛虫科昆虫的一种水溶性毒素进行了鉴定。通过HPLC、小鼠毒性和[H-3]石房蛤毒素与脑膜结合的抑制，估计每只青蛙皮肤的河豚毒素量为0.1至1.2 μ g。还存在少量脱水河豚毒素和4-表河豚毒素。河豚毒素样活性没有检测到抑制[H-3]石房蛤毒素结合在其他物种的Colostethus，也没有在其他dendrobatis（Aromobates，Dendrobates，Phyllobates）。河豚毒素样活性存在于五种Atelopus（蟾蜍科）的皮肤提取物。经HPLC分析，河豚毒素为泡状Atelopus spumarius和A. varius，为A. spurrelli，并作为A. ignescens和A. zeteki。在最后三个物种中的主要河豚毒素样化合物未被确定。河豚毒素样活性没有检测到抑制[H-3]石房蛤毒素结合在皮肤提取物从其他三个属的蟾蜍。
The water-soluble toxin present in skin of Colostethus inguinalis (Dendrobatidae) was identified as tetrodotoxin by fluorometric HPLC analysis. The amount of tetrodotoxin per frog skin was estimated by HPLC, mouse toxicity, and inhibition of [H-3]saxitoxin binding to brain membranes as 0.1 to 1.2 mug. Small amounts of anhydrotetrodotoxin and 4-epietrodotoxin also were present. Tetrodotoxin-like activity was not detected by inhibition of [H-3]saxitoxin binding in other species of Colostethus nor in other dendrobatids (Aromobates, Dendrobates, Phyllobates). Tetrodotoxin-like activity was present in extracts of skin of five species of Atelopus (Bufonidae). HPLC analysis identified tetrodotoxin as the major toxic component in Atelopus spumarius and A. varius, as a minor component in A. spurrelli, and as a trace component in A. ignescens and A. zeteki. The major tetrodotoxin-like compounds in the last three species were not identified. Tetrodotoxin-like activity was not detected by inhibition of [H-3]saxitoxin binding in skin extracts from three other genera of bufonids.