NEW SECALE-CEREALE (RYE) DNA DERIVATIVES FOR THE DETECTION OF RYE CHROMOSOME SEGMENTS IN WHEAT
NEW SECALE-CEREALE (RYE) DNA DERIVATIVES FOR THE DETECTION OF RYE CHROMOSOME SEGMENTS IN WHEAT
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作者：
MCINTYRE, CL;PEREIRA, S;APPELS, R
黑麦120-bp家族的克隆pSc 119的亚克隆产生了两个非常有用的探针。pSc119.1检测黑麦特异性分散重复序列家族。它存在于所有七条黑麦染色体上，并与每条染色体的全长杂交，除了一些端粒和核仁组织者区域。相比之下，pSc119.2主要与黑麦染色体的端粒区域杂交，具有一些间质位点。与pSc119.1不同，它检测小麦和黑麦染色体中相似的重复序列家族。
Subcloning of a clone of the 120-bp family of rye, pSc119, has produced two extremely useful probes. pSc119.1 assays rye-specific dispersed repetitive sequence families. It is present on all seven rye chromosomes and hybridizes to the entire length of each chromosome, with the exception of some telomeres and the nucleolar organiser region. pSc119.2, in contrast, hybridizes predominantly to the telomeric regions of rye chromosomes, with some interstitial sites. Unlike pSc119.1, it assays similar repetitive sequence families in both wheat and rye chromosomes.