Distribution of zincergic neurons in the mouse forebrain
Distribution of zincergic neurons in the mouse forebrain
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DOI：
10.1002/cne.20308
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发表时间：
2004-11-08
影响因子：
2.5
通讯作者：
Dyck, RH
中科院分区：
文献类型：
--
作者：
Brown, CE;Dyck, RH
突触释放的锌被认为通过调节兴奋性和抑制性受体以及细胞内信号蛋白在神经元信号传导中起重要作用。因此，释放锌的神经元与学习和记忆以及神经病理过程如癫痫、中风和阿尔茨海默病的突触可塑性有关。为了表征这些神经元的分布，研究人员依赖于一种技术，该技术涉及锌硒晶体从轴突终末到起源的细胞体的逆行运输。然而，这种方法的一个主要问题是，标记的细胞体是模糊的高水平的染色在突触扣，特别是在前脑结构中，这种染色是最强烈的。在这里，我们使用了逆行标记方法的修改，消除了锌的末端染色，从而使这些神经元的清晰和全面的描述。锌能神经元被发现在所有的大脑皮层区域，并安排在一个独特的层状模式，仅限于层2/3，5，和6，在第4层没有标记。在海马中，标记存在于CA 1，CA 3和齿状回，但不在CA 2。在杏仁核、嗅前核、屏状核、顶盖带、内虹膜状区、侧脑室、侧隔、齿状回、上级丘和导水管周围灰质也观察到标记细胞体。此外，逆行标记也注意到在背内侧和外侧下丘脑，以前被认为是缺乏锌能表型的神经元的区域。总的来说，这些数据表明，锌能神经元包括在小鼠前脑神经元的大人口，并将提供一个解剖框架，了解这些神经元在哺乳动物大脑中的功能的重要性。(C)2004 Wiley-Liss，Inc.
Synaptically released zinc is thought to play an important role in neuronal signaling by modulating excitatory and inhibitory receptors and intracellular signaling proteins. Consequently, neurons that release zinc have been implicated in synaptic plasticity underlying learning and memory as well as neuropathological processes such as epilepsy, stroke, and Alzheimer's disease. To characterize the distribution of these neurons, investigators have relied on a technique that involves the retrograde transport of zinc-selenium crystals from axonal boutons to the cell bodies of origin. However, one major problem with this method is that labeling of cell bodies is obscured by high levels of staining in synaptic boutons, particularly within forebrain structures where this staining is most intense. Here, we used a modification of the retrograde labeling method that eliminates terminal staining for zinc, thereby enabling a clear and comprehensive description of these neurons. Zincergic neurons were found in all cerebral cortical regions and were arranged in a distinct laminar pattern, restricted to layers 2/3, 5, and 6 with no labeling in layer 4. In the hippocampus, labeling was present in CA1, CA3, and the dentate gyrus but not in CA2. Labeled cell bodies were also observed in most amygdaloid nuclei, anterior olfactory nuclei, claustrum, tenia tecta, endopiriform region, lateral ventricle, lateral septum, zona incerta, superior colliculus, and periaqueductal gray. Moreover, retrograde labeling was also noted in the dorsomedial and lateral hypothalamus, regions that previously were thought to be devoid of neurons with a zincergic phenotype. Collectively these data show that zincergic neurons comprise a large population of neurons in the murine forebrain and will provide an anatomical framework for understanding the functional importance of these neurons in the mammalian brain. (C) 2004 Wiley-Liss, Inc.