EFFECTS OF INCREASING NUMBERS OF PHAGOCYTIC INCLUSIONS ON HUMAN RETINAL-PIGMENT EPITHELIAL-CELLS IN CULTURE - A MODEL FOR AGING
EFFECTS OF INCREASING NUMBERS OF PHAGOCYTIC INCLUSIONS ON HUMAN RETINAL-PIGMENT EPITHELIAL-CELLS IN CULTURE - A MODEL FOR AGING
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DOI：
10.1136/bjo.70.11.808
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发表时间：
1986-11-01
影响因子：
4.1
通讯作者：
MARSHALL, J
中科院分区：
文献类型：
--
作者：
BOULTON, M;MARSHALL, J
人视网膜色素上皮细胞的培养物已经用多种浓度的生物（脂褐质、黑色素和视杆外节）和非生物（乳胶微球）颗粒进行了挑战。选择颗粒以包括可降解和不可降解系统的实例。通过相差显微镜观察到一系列形态学变化，并且随着浓度的增加，这些变化变得更加不典型。在最高浓度下，细胞摄入了如此多的颗粒，以至于许多细胞死亡，另一些细胞破裂。这些变化的时间过程表明细胞裂解活性和颗粒降解能力之间的关系。该系统作为研究衰老的模型的潜力进行了讨论。
Cultures of human retinal pigment epithelial cells have been challenged with a number of biological (lipofuscin, melanin, and rod outer segments) and non-biological (latex microspheres) particles at a variety of concentrations. The particles were chosen to include examples of both degradable and non-degradable systems. A range of morphological changes were observed by phase contrast microscopy, and these became more atypical with increasing concentration. At the highest concentration cells had ingested so many particles that many had died and others had ruptured. The time course of these changes indicated a relationship between cellular lytic activity and the capacity of the particle to degrade. The potential of this system as a model for studying senescence is discussed.