Proteomic profiling of human hippocampus
Proteomic profiling of human hippocampus
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人类海马体的蛋白质组学分析
作者：
Jae;T. Czech;G. Lubec
海马体是一个重要的大脑区域，在短期记忆的形成中起着核心作用。关于人类海马中蛋白质表达的信息是有限的，因此我们决定生成这个关键大脑结构的蛋白质图谱，从而提供蛋白质构建块和分析工具。应用蛋白质斑点的凝胶内消化的二维电泳，然后结合Mascot搜索程序进行质谱鉴定。使用基质辅助激光解吸/电离飞行时间（MALDI-TOF）或在特殊情况下使用MALDI-TOF/TOF，以保证明确的识别。鉴定了266个蛋白质点，代表165种不同的蛋白质和101种可能的亚型或翻译后修饰。鉴定的蛋白质被分为几个蛋白质组，如代谢，细胞骨架，抗氧化剂，伴侣，信号，转录，蛋白水解，突触体和神经元，假设和杂项结构。这个人类海马蛋白质图谱为亲水性甚至相对小分子量的蛋白质提供了分析工具，并有助于了解蛋白质构建模块，从而为健康和疾病中该大脑区域的蛋白质化学工作奠定了基础。
The hippocampus is an important brain region playing a central role in short‐term memory formation. Information on protein expression in human hippocampus is limited and we therefore decided to generate a protein map of this pivotal brain structure thus providing protein building blocks and an analytical tool. Two‐dimensional electrophoresis with in‐gel digestion of protein spots followed by mass spectrometrical identification coupled to the Mascot search program was applied. Matrix‐assisted laser desorption/ionization‐time of flight (MALDI‐TOF) or in special cases MALDI‐TOF/TOF was used in order to warrant unambiguous identification. 266 protein spots representing 165 different proteins and 101 probable isoforms or post‐translational modifications were identified. The identified proteins were categorized into several protein groups as metabolic, cytoskeleton, antioxidant, chaperone, signaling, transcriptional, proteolytic, synaptosomal and neuronal, hypothetical and miscellaneous structures. This human hippocampus protein map provides an analytical tool for hydrophilic and even relatively small‐molecular‐weight proteins and contributes to the knowledge on protein building blocks thus forming the basis for protein‐chemical work in this brain area in health and disease.