Comparative maps of human 19p13.3 and mouse chromosome 10 allow identification of sequences at evolutionary breakpoints
Comparative maps of human 19p13.3 and mouse chromosome 10 allow identification of sequences at evolutionary breakpoints
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DOI：
10.1101/gr.145200
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发表时间：
2000-09-01
期刊：
影响因子：
7
通讯作者：
Burmeister, M
中科院分区：
文献类型：
--
作者：
Puttagunta, R;Gordon, LA;Burmeister, M
建立了人类染色体（HSA） 19p13.3的cosmid/细菌人工染色体（BAC）连续图谱，并在该序列中定位了50多个基因。通过基因定位和脉冲场凝胶电泳（PFGE）分析，将人类19p13.3约4000 kb的基因和匿名ESTs定位到小鼠10号染色体中央图谱上。HSA 19p13.3与小鼠染色体（MMU） 10共线。相反，相邻的近似为1200kb是倒置的。两个基因位于MMU 10上反转后的50kb区域，其次是小鼠17号染色体的同源区域。合成断点和一个反转断点已经定位到人类的序列区域
A cosmid/bacterial artificial chromosome (BAC) contiguous (contig) map of human chromosome (HSA) 19p13.3 has been constructed, and over 50 genes have been localized to the contig. Genes and anonymous ESTs from approximate to 4000 kb of human 19p13.3 were placed on the central mouse chromosome 10 map by genetic mapping and pulsed-field gel electrophoresis (PFGE) analysis. A region of similar to 2500 kb of HSA 19p13.3 is collinear to mouse chromosome (MMU) 10. In contrast, the adjacent approximate to 1200 kb are inverted. Two genes are located in a 50-kb region after the inversion on MMU 10, followed by a region of homology to mouse chromosome 17. The synteny breakpoint and one of the inversion breakpoints has been localized to sequenced regions in human