ALDH1A1 effect on Yan Yellow Cattle preadipocyte differentiation
ALDH1A1 effect on Yan Yellow Cattle preadipocyte differentiation
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DOI：
10.1080/10495398.2019.1679824
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发表时间：
2019-10-23
影响因子：
3.7
通讯作者：
Zhao, Yumin
中科院分区：
文献类型：
--
作者：
Hu, Zhongchang;Wu, Jian;Zhao, Yumin
乙醛脱氢酶1A 1（ALDH 1A 1）是一种胞浆酶，主要催化乙醛氧化为乙酸，参与脂肪细胞生长发育中的分化和基因表达调控。然而，ALDH 1A 1在燕黄牛脂肪细胞前体形成中的生理作用尚不清楚。本研究通过干扰或过表达ALDH 1A 1基因，探讨ALDH 1A 1基因在燕黄牛脂肪细胞分化过程中的特异性调控。结果，我们发现ALDH 1A 1的mRNA表达水平在祖细胞形成过程中显著增加。此外，脂蛋白脂酶（LPL）和转录因子（PPAR γ，C/EBP α）的表达水平也显著增加。ALDH 1A 1基因过表达和RNA干扰分别促进和抑制成熟脂肪细胞的脂质积累和甘油三酯的产生，以及LPL和转录因子（PPAR γ，C/EBP α）的表达。ALDH 1A 1和成脂因子蛋白表达水平的变化与mRNA水平的变化雅阁。总之，我们的研究结果表明，ALDH 1A 1在前脂肪细胞的分化中起着重要的调节作用。
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cytosolic enzyme that mainly catalyzes the oxidation of acetaldehyde into acetic acid and participates in the regulation of differentiation and gene expression in fat cell growth and development. However, the physiological role of ALDH1A1 in the formation of fat cell precursors in the Yan Yellow Cattle is still not clear. Herein, we investigated the specific regulation of the gene encoding for ALDH1A1 during the differentiation process of the adipocyte cells of the Yan Yellow Cattle by interfering or overexpressing the ALDH1A1 gene. As a result, we found that the mRNA expression levels of ALDH1A1 were significantly increased during the formation of progenitor cells. In addition, the expression levels of the Lipoprotein lipase (LPL) and transcription factors (PPAR gamma, C/EBP alpha) were also significantly increased. ALDH1A1 gene overexpression and RNA interfering promoted and inhibited respectively the lipid accumulation and triglyceride production in mature adipocytes, and the expression of the LPL and transcription factors (PPAR gamma, C/EBP alpha). The changes in the protein expression levels of ALDH1A1 and adipogenic factors were in accord with the changes observed in the mRNA levels. In conclusion, our results indicate that ALDH1A1 plays an important regulatory role in the differentiation of preadipocyte cells.