Dispensable, Redundant, Complementary, and Cooperative Roles of Dopamine, Octopamine, and Serotonin in Drosophila melanogaster
Dispensable, Redundant, Complementary, and Cooperative Roles of Dopamine, Octopamine, and Serotonin in Drosophila melanogaster
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DOI：
10.1534/genetics.112.142042
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发表时间：
2013-01-01
期刊：
影响因子：
3.3
通讯作者：
Krantz, David E.
中科院分区：
文献类型：
--
作者：
Chen, Audrey;Ng, Fanny;Krantz, David E.
为了研究生物胺对黑腹果蝇行为的调控，我们利用了囊泡单胺转运体（VMAT）对所有单胺神经递质的囊泡储存和胞吐释放的广泛需求。我们使用果蝇VMAT（dVMAT）缺失突变体来全面消除胞吐胺释放，然后使用转基因拯救技术在单个或多个胺能系统中恢复dVMAT活性。我们发现幼虫的存活、幼虫的运动以及雌性的生育能力主要依赖于章鱼胺能回路，而血清素或多巴胺的囊泡释放几乎没有明显的输入作用。相比之下，雄性求偶和生育能力可以通过在章鱼胺能或多巴胺能神经元中表达dVMAT来拯救，这表明可能存在冗余回路。成年果蝇运动和惊吓行为的主要方面的拯救需要章鱼胺，但也观察到血清素具有互补作用。有趣的是，成年果蝇的昼夜节律行为不能通过在单一亚型的胺能神经元中表达dVMAT来拯救，而是至少需要两个系统，这表明可能存在意想不到的协同相互作用。利用该模型进行的进一步实验将有助于确定多个胺能系统如何对其他行为的调控起作用。我们的数据还凸显了由标准胞吐释放调控的行为与由其他机制调控的行为之间的潜在差异。
To investigate the regulation of Drosophila melanogaster behavior by biogenic amines, we have exploited the broad requirement of the vesicular monoamine transporter (VMAT) for the vesicular storage and exocytotic release of all monoamine neurotransmitters. We used the Drosophila VMAT (dVMAT) null mutant to globally ablate exocytotic amine release and then restored DVMAT activity in either individual or multiple aminergic systems, using transgenic rescue techniques. We find that larval survival, larval locomotion, and female fertility rely predominantly on octopaminergic circuits with little apparent input from the vesicular release of serotonin or dopamine. In contrast, male courtship and fertility can be rescued by expressing DVMAT in octopaminergic or dopaminergic neurons, suggesting potentially redundant circuits. Rescue of major aspects of adult locomotion and startle behavior required octopamine, but a complementary role was observed for serotonin. Interestingly, adult circadian behavior could not be rescued by expression of DVMAT in a single subtype of aminergic neurons, but required at least two systems, suggesting the possibility of unexpected cooperative interactions. Further experiments using this model will help determine how multiple aminergic systems may contribute to the regulation of other behaviors. Our data also highlight potential differences between behaviors regulated by standard exocytotic release and those regulated by other mechanisms.