THE ULTRASENSITIVE SILVER PROTEIN STAIN ALSO DETECTS NANOGRAMS OF NUCLEIC-ACIDS
THE ULTRASENSITIVE SILVER PROTEIN STAIN ALSO DETECTS NANOGRAMS OF NUCLEIC-ACIDS
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DOI：
10.1016/0006-291x(81)91487-x
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
WANG, K
中科院分区：
文献类型：
--
作者：
SOMERVILLE, LL;WANG, K
针对蛋白质开发的超灵敏银染色程序还可以染色纳克量的[大肠杆菌] RNA和[噬菌体λ]聚丙烯酰胺凝胶中的DNA。描述了一种梯度聚丙烯酰胺凝胶系统，其分离104-106 MW的蛋白质、5S-23 S的RNA和0.4-21 Kb的DNA [内切酶]。核酸银染色在该凝胶系统中的灵敏度大大超过常用的DNA和RNA染料结合菌株。
The ultrasensitive silver staining procedure developed for proteins also stains nanogram quantities of [Escherichia coli] RNA and [phage .lambda.] DNA in polyacrylamide gels. A gradient polyacrylamide gel system is described which separates proteins from 104-106 MW, RNA from 5S-23S and DNA from 0.4-21 Kb [kilobases]. The sensitivity of nucleic acid silver staining in this gel system considerably exceeds that of commonly used DNA and RNA dye-binding strains.