Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system
Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system
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DOI：
10.1016/j.yexcr.2003.09.024
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发表时间：
2004-02-01
影响因子：
3.7
通讯作者：
Moriuchi, T
中科院分区：
文献类型：
--
作者：
Takahashi, Y;Hamada, J;Moriuchi, T
HOX基因是胚胎发育的主控基因。我们假设HOX基因给予细胞空间信息以维持成人体内的组织或器官特异性，并且HOX基因表达失调导致肿瘤发展。我们建立了一个全面的分析系统，定量表达的39人HOX基因的实时逆转录PCR（RT-PCR）方法的基础上。利用该系统对20个正常成体器官的39个HOX基因进行分析，发现5'端HOX基因在尾侧器官中表达，且尾侧器官越多，表达的HOX基因越多。研究还发现，HOX基因的表达模式在同一簇上的相邻基因中比在属于同一旁系同源物的基因中更相似。与正常甲状腺组织相比，甲状腺癌细胞系中某些HOX基因的表达发生了改变，尤其是Abd-B同源盒家族基因。我们的研究结果表明，HOX基因在成人体内的器官特异性表达，Abd-B家族基因的表达失调与甲状腺肿瘤的发生有关。(C)2003年爱思唯尔公司All rights reserved.
HOX genes are well known as master control genes in embryonic morphogenesis. We hypothesized that HOX genes give cells spatial information to maintain tissue- or organ-specificity in adult body and that the deregulated expression of HOX genes results in tumor development. We established a comprehensive analysis system to quantify expression of 39 human HOX genes based on the real-time reverse transcription PCR (RT-PCR) method. Analysis of 39 HOX genes of 20 normal adult organs by this system revealed that 5' HOX genes were expressed in organs in the caudal parts of the body, and that the more caudal regions the more numbers of HOX genes were expressed. It was also found that the expression patterns of HOX genes were more similar in the adjacent genes on the same cluster rather than in those belonging to the same paralogs. Compared with normal thyroid tissues, thyroid cancer cell lines showed the altered expression of some HOX genes, especially Abd-B homeobox family genes. Our results showed that HOX genes were organ-specifically expressed in adult body and that the deregulated expressions of Abd-B family genes were implicated in thyroid tumor development. (C) 2003 Elsevier Inc. All rights reserved.