Antioxidant effects of herbal therapies used by patients with inflammatory bowel disease:: an in vitro study
Antioxidant effects of herbal therapies used by patients with inflammatory bowel disease:: an in vitro study
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DOI：
10.1046/j.1365-2036.2002.01157.x
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发表时间：
2002-02-01
影响因子：
7.6
通讯作者：
Rampton, DS
中科院分区：
文献类型：
--
作者：
Langmead, L;Dawson, C;Rampton, DS
背景：患者用于治疗炎症性肠病的草药包括滑榆树、胡芦巴、魔爪、墨西哥山药、托美地尔和传统中药胃痛宁。炎症的结肠粘膜产生的活性氧代谢产物可能是致病的。氨基水杨酸酯(5-ASA)是一种抗氧化剂，其他类似药物可用于治疗。目的：评价中药制剂在无细胞氧化剂生成系统和炎症性大肠活检组织中的抗氧化作用。方法：采用黄嘌呤/黄嘌呤氧化酶无细胞体系中的鲁米诺增强化学发光法检测中草药和5-ASA对超氧阴离子的清除作用，用藻红蛋白降解试验用荧光法确定过氧化自由基的清除作用。采用化学发光法检测中药对活动期溃疡性结肠炎患者粘膜组织氧自由基生成的影响。结果：与5-ASA一样，除胡芦巴外，其余中药均能清除超氧阴离子自由基，且呈剂量依赖关系。所有受试材料均能清除过氧化氢，且呈剂量依赖关系。除墨西哥山药外，所有中草药与5-AsA孵育后，活检组织氧自由基释放量均减少。结论：6种中草药均具有抗氧化作用。胡芦巴不是超氧化物清除剂，而墨西哥山药也不能抑制炎症组织产生的自由基。滑榆叶、胡芦巴、魔爪、扭体醇、胃痛宁作为治疗炎症性肠病的新疗法值得正式评价。
Background: Herbal remedies used by patients for treatment of inflammatory bowel disease include slippery elm, fenugreek, devil's claw, Mexican yam, tormentil and wei tong ning, a traditional Chinese medicine. Reactive oxygen metabolites produced by inflamed colonic mucosa may be pathogenic. Aminosalicylates (5-ASA) are antioxidant and other such agents could be therapeutic.Aims: To assess the antioxidant effects of herbal remedies in cell-free oxidant-generating systems and inflamed human colorectal biopsies.Methods: Luminol-enhanced chemiluminescence in a xanthine/xanthine oxidase cell-free system was used to detect superoxide scavenging by herbs and 5-ASA, and fluorimetry to define peroxyl radical scavenging using a phycoerythrin degradation assay. Chemiluminescence was used to detect herbal effects on generation of oxygen radicals by mucosal biopsies from patients with active ulcerative colitis.Results: Like 5-ASA, all herbs, except fenugreek, scavenged superoxide dose-dependently. All materials tested scavenged peroxyl dose-dependently. Oxygen radical release from biopsies was reduced after incubation in all herbs except Mexican yam, and by 5-ASA.Conclusions: All six herbal remedies have antioxidant effects. Fenugreek is not a superoxide scavenger, while Mexican yam did not inhibit radical generation by inflamed biopsies. Slippery elm, fenugreek, devil's claw, tormentil and wei tong ning merit formal evaluation as novel therapies in inflammatory bowel disease.