Chronic intraventricular administration of cholecystokinin octapeptide (CCK-8) suppresses feeding in rats.
Chronic intraventricular administration of cholecystokinin octapeptide (CCK-8) suppresses feeding in rats.
复制标题
慢性心室内注射胆囊收缩素八肽（CCK-8）可抑制大鼠进食。
DOI：
10.1016/0006-8993(88)91266-8
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Go,VL
中科院分区：
文献类型：
--
作者：
Schick,RR;Stevens,CW;Yaksh,TL;Go,VL
八肽缩胆囊素(CCK-8)可抑制急性脑室内注射(i.c.v)后的绵羊、猪、金黄地鼠和大鼠的摄食。注射。在这项研究中，我们报告了长期静脉注射的效果。CCK-8对大鼠长期摄食量的影响。建立基础摄食量3d后，植入剂量为1.0μL/h的阿尔茨海默病渗透压微泵，分成3组，分别给予生理盐水、CCK-8 12.25μg/d(低剂量)或CCK-8 122.5μg/d(高剂量)。手术准备动物的脑室插管和渗透性微泵导致所有组动物最初的食物消耗减少。在生理盐水组，每天的食物摄入量在第4天恢复到手术前的水平。小剂量的CCK-8也观察到了类似的结果。相比之下，在接受高浓度CCK-8的动物中，最初的摄食下降更为明显，尽管在7天的输液间隔期间有所上升，但在此期间从统计上讲仍然低于控制。在停止输液后，在接下来的3天里，每天的食物消耗量上升到正常水平。作为比较，生理盐水组大鼠输液中8d的日摄食量与输液前对照的累积摄食量之差为39.9±10.0g/24 h。在CCK8输注的动物中，低剂量和高剂量分别减少35.5±5.0g/24 h和117.4±20.2g/24 h的积分值。因此，按所有指数计算，I.C.V.与生理盐水相比，大剂量注射CCK-8，而不是小剂量注射CCK-8可显著减少摄食量(P<；0.05)。与生理盐水相比，低剂量或高剂量的CCK-8对小鼠的水摄入量没有影响。
Cholecystokinin octapeptide (CCK-8) is known to suppress feeding in sheep, pigs, golden hamsters and rats following acute intracerebriventricular (i.c.v.) injection. In this study, we report the effects of chronically administered i.c.v. CCK-8 on long-term food intake in rats. After baseline food intake was established over a period of 3 days, rats were implanted with Alzet osmotic minipumps, which delivered 1.0 μl/h. Three groups of animals were prepared which received saline (vehicle) or CCK-8 at 12.25 μg/day (low dose) or CCK-8 at 122.5 μg/day (high dose). Surgical preparation of the animals with the intraventricular cannula and the osmotic minipump resulted in an initial reduction in food consumption in all groups. In the saline group daily food consumption returned to presurgery values by day 4. Similar results were observed with the low dose of CCK-8. In contrast, in animals receiving the high concentrations of CCK-8, the initial fall in feeding was more prominent and though it rose during the 7-day infusion interval, it remained statistically below control during this period. After termination of the infusion, daily food consumption rose to normal levels during the next 3 days. For comparison, the cumulative difference between daily food consumption over the period of 8 days during infusion and pre-infusion control was 39.9 ± 10.0 g/24 h in the saline group. In CCK-8-infused animals, food consumption after pump implantation was reduced by an integrated value of 35.5 ± 5.0 g/24 h at low dose and 117.4 ± 20.2 g/24 h at high dose. Thus, by all indices, i.c.v. infusion of CCK-8 at high dose, but not at low dose resulted in a significant reduction of food intake as compared ti saline (P< 0.05). Water intake was not not altered by the infusion of CCK-8 at low or high dose vs saline.