Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis.
Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis.
复制标题
未能从中国仓鼠卵巢细胞突变体多效性多毒性中释放出铁蛋白在内吞作用中有缺陷。
DOI：
10.1083/jcb.98.3.1098
复制
发表时间：
1984-03
影响因子：
7.8
通讯作者：
Robbins, A R
中科院分区：
文献类型：
--
作者：
Klausner, R D;van Renswoude, J;Kempf, C;Rao, K;Bateman, J L;Robbins, A R
中国仓鼠卵巢细胞突变株(Robbins，A.R.，S.S.Peng，和J.L.Matt，1983，J.Cell Biol.，96：1064-1071)在受体介导的内吞作用中存在缺陷，未能积聚以二铁转铁蛋白形式提供的铁。对转铁蛋白循环步骤的分析表明，突变细胞中转铁蛋白的结合和内化正常进行。然而，突变体似乎不能将铁从转铁蛋白中分离出来，突变体释放的二铁转铁蛋白与亲本的脱铁蛋白相比证明了这一点。突变株对生长介质中铁离子的吸收增强。
A Chinese hamster ovary cell mutant defective in the receptor-mediated endocytosis of several unrelated ligands (Robbins, A. R., S. S. Peng, and J. L. Marshall, 1983, J. Cell Biol., 96:1064-1071) failed to accumulate iron provided in the form of diferric transferrin. Analysis of the steps of the transferrin cycle indicated that binding and internalization of transferrin proceeded normally in mutant cells. However, the mutant appeared unable to dissociate iron from transferrin, as evidenced by release of diferric transferrin from the mutant versus apotransferrin from the parent. Uptake of ferric ions from the growth medium was enhanced in the mutant.