Spectral response of a methylimidazolium-functionalized polythiophene to phosphates
Spectral response of a methylimidazolium-functionalized polythiophene to phosphates
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DOI：
10.1016/j.dyepig.2012.04.012
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发表时间：
2012-11
影响因子：
4.5
通讯作者：
C. Spangler;T. Lang;M. Schäferling
中科院分区：
文献类型：
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作者：
C. Spangler;T. Lang;M. Schäferling
本文报道了阳离子水溶性聚噻吩衍生物聚[3-（2-（N-（N′-甲基咪唑））乙氧基）-4-甲基噻吩]在不同磷酸盐存在下的比色和荧光响应。UV/维斯吸光度和荧光测量表明，可以在水溶液中测定ATP浓度，对其他磷酸盐具有足够的选择性。圆二色性的测量表明，聚合物形成无规卷曲结构，在CD光谱中没有信号。加入ATP后，构象变为螺旋，产生强CD峰。这些实验数据表明，这种聚合物是一个有吸引力的，简单的和通用的比色和荧光探针核苷三磷酸，如ATP。
We report on the colorimetric and fluorimetric responses of the cationic water-soluble polythiophene derivative poly[3-(2-(N-(N′-methylimidazole))ethoxy)-4-methylthiophene] in presence of different phosphates with a focus on ATP. UV/Vis absorbance and fluorescence measurements show that ATP concentrations can be determined in aqueous solutions with a sufficient selectivity to other phosphates. Measurements of circular dichroism reveal that the polymer forms a random coil structure that exhibits no signal in the CD spectrum. Upon addition of ATP the conformation changes to a helix, yielding in strong CD peaks. These experimental data suggest that this polymer is an attractive, simple and versatile colorimetric and fluorimetric probe for nucleoside triphosphates such as ATP.