Feed intake, feed utilization and feeding-related gene expression response to dietary phytic acid for juvenile grass carp (Ctenopharyngodon idellus)
Feed intake, feed utilization and feeding-related gene expression response to dietary phytic acid for juvenile grass carp (Ctenopharyngodon idellus)
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草鱼幼鱼（Ctenopharyngodon idellus）的采食量、饲料利用率以及对日粮植酸的摄食相关基因表达反应
DOI：
10.1016/j.aquaculture.2013.12.044
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发表时间：
2014-03-20
期刊：
影响因子：
4.5
通讯作者：
He, Yan
中科院分区：
文献类型：
--
作者：
Liu, Liwei;Liang, Xu-Fang;He, Yan
饲料植酸（PA）对摄食量的负面影响是鱼类应激反应的一个共同特征，但对摄食量的调节机制知之甚少。本研究旨在探讨饲料中添加PA对草鱼幼鱼摄食量、饲料利用率及摄食相关基因表达的影响。日粮PA补充水平分别为0（对照）、5（低）和40（高）g kg（-1）日粮。草鱼（平均体重为22.37 ± 0.16 g）的三个组（9个300 L水槽）每天投喂两次（08：00和16：00 h）至饱腹，共投喂8周。结果表明，添加PA降低了草鱼的增重、摄食量、饲料利用率和消化酶活性。日粮中添加PA降低了磷、钙和粗蛋白的表观消化率。可卡因和安非他明调节转录物（CART）和胆囊收缩素（CCK）在脑中的基因表达水平随着膳食PA补充量的增加而增强。与对照组相比，低PA组神经肽Y（NPY）和ghrelin mRNA表达水平降低，而高PA组显著升高（P < 0.05）。NPY受体Y8 a和Y8 b基因表达在各组间差异无统计学意义。本研究结果表明，饲料利用率的降低以及脑组织CART和CCK基因表达的增加可能是饲料中添加PA导致草鱼摄食量降低的主要原因。皇冠版权所有（C）2014由爱思唯尔B. V.出版保留所有权利。
The negative effect of dietary phytic acid (PA) in feed intake is a common feature of response to stress in fish, but the regulation mechanism of feed intake is poorly understood. Our study was therefore conducted to estimate the effects of dietary PA on feed intake, feed utilization and feeding related gene expression in juvenile grass carp. The levels of dietary PA supplementation were 0 (control), 5 (low) and 40 (high) g kg(-1) diet, respectively. Triplicate groups (nine 300-L tanks) of grass carp (mean weight, 22.37 +/- 0.16 g) were fed twice daily (08:00 and 16:00 h) to satiation for 8 weeks. Supplemental PA decreased the weight gain, feed intake, feed utilization and digestive enzyme activities of grass carp. Dietary PA supplementation decreased the apparent digestibilities of phosphorus, calcium and crude protein. The gene expression levels of cocaine- and amphetamine-regulated transcript (CART) and cholecystokinin (CCK) in the brain were enhanced with the increase of dietary PA supplementation. However, the neuropeptide Y (NPY) and ghrelin mRNA expression levels were reduced in fish fed with low PA, but increased significantly (P < 0.05) in fish fed with high PA compared to the control. No significant differences were observed in the gene expressions of NPY receptors Y8a and Y8b among all the groups. The results of this study indicated that the decrease of feed utilization and the increase of CART and CCK gene expressions in the brain might be the main factors for the decrease of teed intake in grass carp caused by dietary PA supplementation. Crown Copyright (C) 2014 Published by Elsevier B.V. All rights reserved.