Dual oxidase in the intestinal epithelium of zebrafish larvae has anti-bacterial properties
Dual oxidase in the intestinal epithelium of zebrafish larvae has anti-bacterial properties
复制标题
DOI：
10.1016/j.bbrc.2010.08.037
复制
发表时间：
2010-09-10
影响因子：
3.1
通讯作者：
Crosier, Philip S.
中科院分区：
文献类型：
--
作者：
Flores, Maria Vega;Crawford, Katie C.;Crosier, Philip S.
活性氧（Reactive oxygen species，ROS）不仅在生长、代谢和信号传导等生理过程中发挥作用，而且还具有病理作用。最近的研究强调了在先天性免疫和炎症性肠病（IBD）等炎症性疾病的宿主防御反应中需要双氧化酶（DUOX）产生的ROS，但迄今为止，缺乏支持这一点的体内证据。为了研究Duox在肠道免疫中的参与，我们表征了人DUOX基因的斑马鱼直系同源物。斑马鱼duox在肠上皮细胞中高度表达。Duox的敲除损害了幼虫控制肠道沙门氏菌感染的能力。(C)2010年爱思唯尔公司All rights reserved.
Reactive oxygen species (ROS) function in a range of physiological processes such as growth, metabolism and signaling, and also have a pathological role. Recent research highlighted the requirement for ROS generated by dual oxidase (DUOX) in host-defence responses in innate immunity and inflammatory disorders such as inflammatory bowel disease (IBD), but in vivo evidence to support this has, to date, been lacking. In order to investigate the involvement of Duox in gut immunity, we characterized the zebrafish ortholog of the human DUOX genes. Zebrafish duox is highly expressed in intestinal epithelial cells. Knockdown of Duox impaired larval capacity to control enteric Salmonella infection. (C) 2010 Elsevier Inc. All rights reserved.