CHRONIC EXPOSURE OF CULTURED ENDOTHELIAL-CELLS TO EICOSAPENTAENOIC ACID POTENTIATES THE RELEASE OF ENDOTHELIUM-DERIVED RELAXING FACTOR(S)
CHRONIC EXPOSURE OF CULTURED ENDOTHELIAL-CELLS TO EICOSAPENTAENOIC ACID POTENTIATES THE RELEASE OF ENDOTHELIUM-DERIVED RELAXING FACTOR(S)
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DOI：
10.1111/j.1476-5381.1990.tb14673.x
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发表时间：
1990-01-01
影响因子：
7.3
通讯作者：
VANHOUTTE, PM
中科院分区：
文献类型：
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作者：
BOULANGER, C;SCHINI, VB;VANHOUTTE, PM
1 培养的猪主动脉内皮细胞长期暴露于二十碳五烯酸对吲哚美辛不敏感舒张因子释放的影响进行了研究（a）在生物测定条件下使用无内皮的预收缩犬冠状动脉环和（b）通过测量内皮细胞的鸟苷 3''：5''-环磷酸（环 GMP）含量。 2 内皮细胞暴露于二十碳五烯酸（2.5×10-5M）8-10天不影响来自未刺激内皮细胞的灌注液的松弛活性。 3 用二磷酸腺苷（3 × 10-8 M 至 3 × 10-4 M）刺激内皮细胞时观察到，二十碳五烯酸处理显着增加了生物测定环的松弛，并且用缓激肽（10-8 至 3 × 10-8 M）刺激内皮细胞时观察到的生物测定环的松弛程度较小，而钙离子载体引起的松弛活性A23187（3×10-7 M）不受影响。乙酰胆碱 (10-6 M) 和 5-羟色胺 (10-6 m) 均不会刺激对照或二十碳五烯酸处理的内皮细胞释放松弛因子。 4. 缓激肽 (10-7M)k、二磷酸腺苷 (3 7x 10-5 M)、钙肌力 A23187 (10-6 M) 和一氧化氮 (2 × 10-6 M) 刺激猪内皮细胞中血红蛋白敏感的环 GMP 含量增加，而猪内皮细胞不受先前慢性暴露于二十碳五烯酸的影响酸。 5.这些结果表明，猪主动脉内皮细胞长期暴露于二十碳五烯酸会增加响应膜相关嘌呤和激肽受体激活的松弛因子的释放。二十碳五烯酸处理对激动剂刺激的环 GMP 产生没有影响，这表明在生物测定条件下观察到的松弛增强并不是由于一氧化氮产生的增加。
1 The effect of chronic exposure of culture porcine aortic endothelial cells to eicosapentaenoic acid on the release of indomethacin-insensitive relaxing factor(s) was investigated (a) under bioassay conditions using preconstricted canine coronary artery rings without endothelium and (b) by the measurement of guanosine 3'':5''-cyclic monophosphate (cyclic GMP) content of endothelial cells. 2 Exposure of endothelial cells for 8-10 days to eicosapentaenoic acid (2.5 .times. 10-5 M) did not affect the relaxing activity of the perfusate from unstimulated endothelial cells. 3 The treatment with eicosapentaenoic acid significantly increased the relaxation of the bioassay ring observed upon stimulation of the endothelial cells with adenosine diphosphate (3 .times. 10-8 M to 3 .times. 10-4 M) and, to a lesser extent with bradykinin (10-8 to 3 .times. 10-8 M), while the relaxing activity evoked by the calcium ionophore A23187 (3 .times. 10-7 M) was not affected. Neither acetylcholine (10-6 M) nor 5-hydroxytryptamine (10-6 m) stimulated the release of relaxing factor(s) from control or eicosapentaenoic acid-treated endothelial cells. 4. Bradykinin (10-7M)k, adenosine diphosphate (3 7x 10-5 M), the calcium inophore A23187 (10-6 M) and nitric oxide (2 .times. 10-6 M) stimulated haemoglobin-sensitive increases in cyclic GMP content of porcine endothelial cells which were unaffected by prior chornic exposure to eicospentaenoic acid. 5. These results suggest that chronic exposure of porcine aortic endothelial cells to eicosapentaenoic acid increases the release of relaxing factor(s) in response to activation of membrane-associated receptors for purines and kinins. The lack of effect of eicosapentaenoic acid treatment on agonist-stimulated production of cyclic GMP suggests that the enhanced relaxation observed under bioassay conditions is not due to an increased production of nitric oxide.