Spermatogonial stem cell markers and niche in equids.
Spermatogonial stem cell markers and niche in equids.
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DOI：
10.1371/journal.pone.0044091
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Franca LR
中科院分区：
文献类型：
--
作者：
Costa GM;Avelar GF;Rezende-Neto JV;Campos-Junior PH;Lacerda SM;Andrade BS;Thomé RG;Hofmann MC;Franca LR
精原干细胞（Spermatogonial stem cells，SSCs）是精子发生的基础，位于一个高度动态的微环境中，影响着干细胞功能的各个方面，包括归巢、自我更新和分化。最近有几项研究确定了调节SSC命运的特定蛋白质。这些研究还旨在鉴定有助于在不同脊椎动物物种中分离这些细胞的表面标记。本研究是第一个调查SSC的生理和生态位的公马，并提供了一个比较评估未分化的A型精原细胞（Aund）标记（GFRA 1，PLZF和CSF 1 R）在三个不同的国内马种（公马，驴，骡子）。Aund首先根据其形态和GFRA 1受体的表达进行表征。我们的研究结果强烈表明，在种马，这些细胞优先位于面向的区域，特别是那些附近的血管。这种分布与在其他脊椎动物物种中观察到的相似。此外，所有三种Aund标记物均在本研究中评价的马属物种中表达。这些标记物在其他哺乳动物物种中已得到很好的表征，这表明维持生态位和Aund/SSC生理学的分子机制在哺乳动物中是保守的。我们希望我们的研究结果将有助于未来的研究需要分离和冷冻保存的马精原干细胞。此外，我们的数据将是非常有用的研究，旨在保存有价值的动物的种质，并涉及生殖细胞移植或异种移植的马睾丸碎片/生殖细胞悬浮液。
Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis and are located in a highly dynamic microenvironment called “niche” that influences all aspects of stem cell function, including homing, self-renewal and differentiation. Several studies have recently identified specific proteins that regulate the fate of SSCs. These studies also aimed at identifying surface markers that would facilitate the isolation of these cells in different vertebrate species. The present study is the first to investigate SSC physiology and niche in stallions and to offer a comparative evaluation of undifferentiated type A spermatogonia (Aund) markers (GFRA1, PLZF and CSF1R) in three different domestic equid species (stallions, donkeys, and mules). Aund were first characterized according to their morphology and expression of the GFRA1 receptor. Our findings strongly suggest that in stallions these cells were preferentially located in the areas facing the interstitium, particularly those nearby blood vessels. This distribution is similar to what has been observed in other vertebrate species. In addition, all three Aund markers were expressed in the equid species evaluated in this study. These markers have been well characterized in other mammalian species, which suggests that the molecular mechanisms that maintain the niche and Aund/SSCs physiology are conserved among mammals. We hope that our findings will help future studies needing isolation and cryopreservation of equids SSCs. In addition, our data will be very useful for studies that aim at preserving the germplasm of valuable animals, and involve germ cell transplantation or xenografts of equids testis fragments/germ cells suspensions.