Ovalbumin-induced neurogenic inflammation in the bladder of sensitized rats
Ovalbumin-induced neurogenic inflammation in the bladder of sensitized rats
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DOI：
10.1038/sj.bjp.0701793
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发表时间：
1998-05-01
影响因子：
7.3
通讯作者：
Maggi, CA
中科院分区：
文献类型：
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作者：
Ahluwalia, A;Giuliani, S;Maggi, CA
1我们建立并鉴定了一种局部应用卵白蛋白(OA)致雌性大鼠膀胱即刻超敏/炎症反应的模型。评估了OA激发后炎症反应的两个参数：血浆蛋白外渗(PPE)和平滑肌反应性的变化。通过测定活体激发后0.5、2、4、8和24 h的伊文思蓝渗出量来评估前者。2在主动致敏的雌性Wistar大鼠膀胱内急性OA激发(10 mg生理盐水)引起膀胱PPE呈时间依赖性，高峰出现在2~4和24小时。3辣椒素(50 mg·kg~(-1)，sc，用药前4天)可取消2 h时对急性OA激发的PPE(高于基线)(P
1 We have developed and characterized a model of immediate hypersensitivity/inflammation of the urinary bladder in vivo induced by local application of ovalbumin (OA) in OA-sensitive female rats. Two parameters of the inflammatory response were assessed following OA challenge: plasma protein extravasation (PPE) and changes in smooth muscle reactivity. The former was estimated by measurement of Evans blue extravasation at 0.5, 2, 4, 8 and 24 h time point following in vivo challenge. Changes in reactivity were determined by measurement of isotonic tension responses of urinary bladder strips following OA challenge in vitro.2 Acute in vivo intravesical OA challenge (10 mg in 0.3 mi saline) in actively sensitized female Wistar rats caused a time-dependent PPE in the urinary bladder which was biphasic with peak responses at 2-4 and 24 h.3 The PPE response to acute OA challenge, above base-line, at 2 h was abolished by systemic capsaicin pretreatment (50 mg kg(-1), s.c., 4 days before use) (P