Association of chemokine and chemokine receptor expression with the invasion and metastasis of lung carcinoma
Association of chemokine and chemokine receptor expression with the invasion and metastasis of lung carcinoma
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DOI：
10.3892/ol.2015.3402
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发表时间：
2015-09-01
期刊：
影响因子：
2.9
通讯作者：
Zhong, Hao-Hao
中科院分区：
文献类型：
--
作者：
Liu, Yan;Wu, Bing-Quan;Zhong, Hao-Hao
据报道，趋化因子系统被肿瘤细胞利用和操纵，以促进肿瘤的局部生长和远处传播。本研究旨在探讨三种趋化因子配体受体轴在肺癌组织中的表达。本文从120例肺癌患者手术切除后获得肿瘤和健康正常组织标本。采用免疫组织化学和逆转录定量聚合酶链反应鉴定趋化因子(趋化因子（C-X-C motif）配体（CXCL）12/基质细胞衍生因子(SDF)-1、CXCL8/白细胞介素(IL)-8、趋化因子（C-C motif）配体（CCL）19和CCL21，以及相应的趋化因子受体、趋化因子（C-X-C motif）受体（CXCR）4、CXCR1、CXCR2和趋化因子（C-C motif）受体（CCR）7的蛋白和信使(m)RNA表达。结果显示，与正常肺组织相比，肺癌组织中CXCL12/SDF-1、CXCR4、CXCL8/IL-8、CXCR2、CCL19和CCR7 mRNA表达水平显著升高(P
The chemokine system has been reported to be utilized and manipulated by tumor cells in order to promote local tumor growth and distant dissemination. The present study aimed to investigate the expression of three chemokine ligand-receptor axes in lung carcinoma tissues. Tumor and healthy normal tissue samples were obtained from 120 lung carcinoma patients following surgical resection. Immunohistochemistry and reverse transcription quantitative polymerase chain reaction were used in order to identify the protein and messenger (m)RNA expression of chemokines, including chemokine (C-X-C motif) ligand (CXCL)12/stromal cell-derived factor (SDF)-1, CXCL8/interleukin (IL)-8, chemokine (C-C motif) ligand (CCL)19 and CCL21, and the corresponding chemokine receptors, chemokine (C-X-C motif) receptor (CXCR)4, CXCR1, CXCR2 and chemokine (C-C motif) receptor (CCR)7, respectively. The results revealed that compared with the normal lung tissues, lung carcinoma tissues expressed significantly higher mRNA levels of CXCL12/SDF-1, CXCR4, CXCL8/IL-8, CXCR2, CCL19 and CCR7 (P