Detection of residual acute lymphoblastic leukemia cells in cultures of bone marrow obtained during remission.
Detection of residual acute lymphoblastic leukemia cells in cultures of bone marrow obtained during remission.
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检测缓解期间获得的骨髓培养物中残留的急性淋巴细胞白血病细胞。
DOI：
10.1056/nejm198608283150902
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Freedman
中科院分区：
文献类型：
--
作者：
Z. Estrov;T. Grunberger;I. Dubé;Y. P. Wang;M. Freedman
我们采用半固体培养法对儿童急性淋巴细胞白血病（ALL）患者骨髓中的白血病细胞进行定量。在40例新诊断疾病患者的骨髓培养物中，体外培养的集落由具有与原始诊断骨髓标本相同的表面标记和异常核型的成淋巴细胞组成。我们还研究了13例化疗诱导缓解患者的骨髓培养物;其中6例，包括1例从骨髓移植后成功植入的患者中获得的，也在培养中产生了淋巴母细胞集落，具有与原始白血病骨髓相同的免疫表型或异常核型。其中四名患者，包括接受骨髓移植的患者，在异常培养物的2至30个月内复发;另外两名患者仍在缓解中，其中一名患者在诊断后30个月。8名正常对照和其他7名缓解期患者的骨髓培养物未产生淋巴母细胞集落;所有7名缓解期患者仍处于缓解期。该测定似乎允许检测少量残留白血病细胞。我们的结论是，该技术将是有价值的监测化疗和异基因骨髓移植治疗急性淋巴细胞白血病的疗效，以及在评估净化骨髓自体骨髓移植的质量。
We used a semisolid culture assay to quantitate leukemia cells in the bone marrow of patients with childhood acute lymphoblastic leukemia (ALL). In bone marrow cultures from 40 patients with newly diagnosed disease, the colonies that developed in vitro consisted of lymphoblasts with the same surface markers and abnormal karyotype as the original diagnostic marrow specimens. We also studied marrow cultures from 13 patients in chemotherapy-induced remission; 6 of these, including 1 obtained from a patient during successful engraftment after marrow transplantation, also yielded lymphoblast colonies in culture, with the same immunologic phenotype or abnormal karyotype as the original leukemic marrow. Four of these patients, including the one who underwent marrow transplantation, relapsed within 2 to 30 months of the abnormal cultures; the other two are still in remission, one of them 30 months after diagnosis. Bone marrow cultures from eight normal controls and from the other seven patients in remission did not yield lymphoblast colonies; all seven of the latter are still in remission. This assay appears to allow detection of small numbers of residual leukemic cells. We conclude that the technique will be valuable in monitoring the efficacy of chemotherapy and allogeneic bone marrow transplantation in acute lymphoblastic leukemia, as well as in evaluating the quality of purged marrow for autologous marrow transplantation.
对第二次或随后缓解的急性淋巴细胞白血病患者进行强化治疗后进行骨髓移植：预后因素的确定（明尼苏达大学骨髓移植小组的报告）。
影响因子：
20.3
作者：
Woods,WG;Nesbit,ME;Ramsay,NK;Krivit,W;Kim,TH;Goldman,A;McGlave,PB;Kersey,JH
通讯作者：
Kersey,JH
急性淋巴细胞白血病第二次或后续缓解患者的自体骨髓移植：单克隆抗体 BA-1、BA-2 和 BA-3 加补体治疗骨髓的结果。
影响因子：
20.3
作者：
Ramsay,N;LeBien,T;Nesbit,M;McGlave,P;Weisdorf,D;Kenyon,P;Hurd,D;Goldman,A;Kim,T;Kersey,J
通讯作者：
Kersey,J
急性淋巴细胞白血病患者的同种异体骨髓移植。
影响因子：
20.3
作者：
Dinsmore,R;Kirkpatrick,D;Flomenberg,N;Gulati,S;Kapoor,N;Shank,B;Reid,A;Groshen,S;O'Reilly,RJ
通讯作者：
O'Reilly,RJ