Differential effect of functional olfactory bulb deafferentation on tyrosine hydroxylase and glutamic acid decarboxylase messenger RNA levels in rodent juxtaglomerular neurons.
Differential effect of functional olfactory bulb deafferentation on tyrosine hydroxylase and glutamic acid decarboxylase messenger RNA levels in rodent juxtaglomerular neurons.
复制标题
功能性嗅球传入神经阻滞对啮齿动物肾小球旁神经元酪氨酸羟化酶和谷氨酸脱羧酶信使 RNA 水平的差异影响。
DOI：
10.1002/cne.903110205
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Baker,H
中科院分区：
文献类型：
--
作者：
Stone,DM;Grillo,M;Margolis,FL;Joh,TH;Baker,H
嗅球旁神经元多巴胺能表型的表达依赖于外周嗅觉受体的功能性神经支配。在啮齿动物中，由于外周去传入或气味通路被剥夺，导致功能性输入的丧失，导致肾小球旁酪氨酸羟基酶(TH)的表达显著降低。我们研究了这些处理对神经递质生物合成酶谷氨酸脱羧酶(GAD)表达的影响，GAD与TH共存于大多数含有TH的球旁神经元中。在化学诱导的成年小鼠去传入或新生大鼠单侧气味剥夺后，经Northern印迹分析，在治疗20-40天后，稳态的OB GAD信使RNA水平基本保持不变。原位杂交分析证实了这些结果，表明肾小球旁TH信使RNA严重缺失，但区域共定位的GAD信息没有伴随的减少。由于GAD几乎存在于所有的多巴胺能OB细胞中，肾小球旁GAD信息的保存表明嗅觉感受器神经元对TH和GAD基因转录起着不同的跨神经元调节作用。
Expression of the dopaminergic phenotype in olfactory bulb (OB) juxtaglomerular neurons (constituting a population of periglomerular and external tufted cells) is dependent upon functional innervation by peripheral olfactory receptors. Loss of functional input in rodents, by either peripheral deafferentation or deprivation of odorant access, results in a profound decrease in the expression of juxtaglomerular tyrosine hydroxylase (TH). We have examined the effects of such treatments on the expression of the neurotransmitter biosynthetic enzyme glutamic acid decarboxylase (GAD), which is colocalized with TH in the majority of TH‐containing juxtaglomerular neurons. Following either chemically induced OB deafferentation in adult mice or unilateral odor deprivation in neonatal rats, steady‐state OB GAD messenger RNA levels remained essentially unchanged as assessed by Northern blot analysis 20–40 days after treatment. These results were confirmed by in situ hybridization analysis, which demonstrated a profound loss of juxtaglomerular TH messenger RNA but no accompanying decrease in regionally colocalized GAD message. Since GAD is found in nearly all dopaminergic OB cells, the preservation of juxtaglomerular GAD message implies that olfactory receptor neurons exert a differential transneuronal regulation of TH and GAD gene transcription.