Mice infected with Schistosoma mansoni generate antibodies to LacdiNAc (GalNAcβ1→4GlcNAc) determinants
Mice infected with Schistosoma mansoni generate antibodies to LacdiNAc (GalNAcβ1→4GlcNAc) determinants
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DOI：
10.1093/glycob/9.10.1029
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发表时间：
1999-10-01
期刊：
影响因子：
4.3
通讯作者：
Cummings, RD
中科院分区：
文献类型：
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作者：
Nyame, AK;Leppanen, AM;Cummings, RD
曼氏血吸虫是一种寄生吸虫，感染人和动物。我们以前报道过曼氏血吸虫成虫合成含有末端序列GalNAcβ1-->；4GlcNAc-R(lacdiNAc或LDN)的复合型双天线N-聚糖。我们现在报告，感染曼氏葡萄球菌的小鼠产生LDN抗体，通过使用含有LDN序列的合成新糖结合物的ELISA进行评估。感染小鼠的血清主要含有IgM，低水平的抗LDN抗体，而非未感染的小鼠。有趣的是，这些抗体还识别牛乳糖蛋白，已知牛乳糖蛋白表达LDN序列。用固定化牛乳糖蛋白亲和纯化感染小鼠血清中的抗LDN，并与LDN特异性结合。从感染曼氏血吸虫小鼠的脾中提取了一种特异性结合LDN决定簇的IgM单抗(SMLDN1.1)。亲和纯化的抗LDN和SMLDN1.1免疫印迹表明，LDN序列主要存在于曼氏血吸虫成虫大量糖蛋白的N-糖链上，在S、日本血吸虫和血吸虫的许多糖蛋白中也有表达，LDN决定簇抗体的存在将有助于确定这些糖蛋白在蠕虫和脊椎动物生物学中的作用。
Schistosoma mansoni is a parasitic trematode infecting humans and animals. We reported previously that adult S.mansoni synthesizes complex-type biantennary N-glycans bearing the terminal sequence GalNAc beta 1-->4GlcNAc-R (lacdiNAc or LDN). We now report that mice infected with S.mansoni generate antibodies to LDN, as assessed by ELISA using a synthetic neoglycoconjugate containing LDN sequences. Sera of infected mice, but not uninfected mice, contained primarily IgM and low levels of IgG toward LDN. Interestingly, these antibodies also recognize bovine milk glycoproteins, which are known to express LDN sequences. The anti-LDN in sera of infected mice were affinity purified on immobilized bovine milk glycoproteins and shown to specifically bind LDN. An IgM monoclonal antibody (SMLDN1.1) was derived from the spleens of S.mansoni infected mice and shown to specifically bind LDN determinants. Immunoblots with affinity purified anti-LDN and SMLDN1.1 demonstrate that LDN sequences occur primarily on N-glycans of numerous glycoproteins of adult S.mansoni, LDN sequences are also expressed in many glycoproteins from S,japonicum and S.haematobium, The availability of antibody to LDN determinants should aid in defining the roles of these glycans in helminth and vertebrate biology.