Covalent layer-by-layer assembly of polyethyleneimine multilayer for antibacterial applications
Covalent layer-by-layer assembly of polyethyleneimine multilayer for antibacterial applications
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DOI：
10.1002/jbm.a.32872
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发表时间：
2010-11-01
影响因子：
4.9
通讯作者：
Chan, Vincent
中科院分区：
文献类型：
--
作者：
He, Tao;Chan, Vincent
聚电解质多层膜（PMF）通常是由一对带相反电荷的聚电解质通过静电吸引在基底上逐层（LBL）组装而成。然而，PMF在生理条件下缺乏长期稳定性限制了其作为医疗器械中的抗菌涂层的应用。在这项研究中，一个稳定的PMF组成的只有聚乙烯亚胺（PEI）的共价LBL沉积构建。首先，通过紫外-可见吸收光谱和原子力显微镜验证了共价LBL组装过程中PEI的特定积聚。第二，银（Ag）纳米粒子被纳入PEI多层膜通过原位还原Ag+的预吸附的硼氢化钠。研究还表明，银纳米粒子的质量可以通过改变多层膜的厚度和加载周期来控制。细菌活/死测定显示PEI多层膜在接触形成后有效地杀死金黄色葡萄球菌和大肠杆菌。在（PEI）膜中包含Ag纳米颗粒不仅增强了对粘附细菌的抗微生物性能，而且通过将Ag+长期释放到液体介质中来抑制悬浮培养物中的细菌生长。(C)2010 Wiley Periodicals，Inc. J Biomed Mater Res Part A：95A：454-464，2010.
Polyelectrolyte multilayer film (PMF) is conventionally fabricated by the layer-by-layer (LBL) assembly of a pair of oppositely charged polyelectrolytes on a substrate through electrostatic attractions. However, the lack of long-term stability of PMF under physiological conditions limits its application as antimicrobial coating in medical devices. In this study, a stable PMF composed of only polyethyleneimine (PEI) was constructed by covalent LBL deposition. First, the specific buildup of PEI during covalent LBL assembly was validated by UV-Vis absorption spectroscopy and atomic force microscopy. Second, silver (Ag) nanoparticles were incorporated into PEI multilayers through in situ reduction of Ag+ by the pre-absorption of NaBH4. It was also shown that the mass of Ag nanoparticle can be controlled by varying multilayer thickness and loading cycles. Bacterial live/dead assay showed that the PEI multilayers effectively killed Staphylococcus aureus and Escherichia coli upon contact formation. The inclusion of Ag nanoparticles in (PEI) film not only enhanced the antimicrobial property against adherent bacteria but also led to the inhibition of the bacteria growth in suspended culture via the long-term release of Ag+ into the liquid medium. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 454-464, 2010.