DNA vaccination against anthrax in mice - combination of anti-spore and anti-toxin components
DNA vaccination against anthrax in mice - combination of anti-spore and anti-toxin components
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DOI：
10.1016/j.vaccine.2005.08.031
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发表时间：
2006-05-22
期刊：
影响因子：
5.5
通讯作者：
Beyer, W
中科院分区：
文献类型：
--
作者：
Hahn, UK;Boehm, R;Beyer, W
人炭疽疫苗中的主要抗原是炭疽芽孢杆菌的保护性抗原（PA）。为了解决这个问题，免疫反应是否对B。炭疽芽孢杆菌外孢壁抗原BcIA（一种胶原样表面蛋白）的真核表达质粒。该质粒pSecTag BcIA携带重组抗原的分泌信号。使用NMRI小鼠，我们比较了用PA和BcIA编码质粒DNA的组合免疫与用PA或BcIA编码pDNA免疫的效果。三次免疫后，用25 × LD 50的B感染小鼠。炭疽艾姆斯孢子。质粒pSecTag BcIA诱导高的BcIA特异性抗体应答。用PA-和BcIA-编码pDNA的组合接种导致比仅用PA-或仅用BcIA-编码质粒的免疫显著更好的存活。(c)2005爱思唯尔有限公司保留所有权利。
The predominant antigen in human anthrax vaccines is the protective antigen (PA) of Bacillus anthracis. To address the question whether immune responses against B. anthracis spores can improve the protectivity of a PA-based DNA vaccine against anthrax, we designed a eucaryotic expression plasmid encoding the exosporium antigen BcIA, which is a collagen-like surface protein. This plasmid, pSecTag BcIA, carries a secretion signal for the recombinant antigen. Using NMRI mice we compared the effects of immunisation with a combination of PA- and BcIA-encoding plasmid DNA, to immunisations with either PA- or BcIA-encoding pDNA. After three immunisations the mice were infected with 25 x LD50 of B. anthracis Ames spores. The plasmid pSecTag BcIA, induced high BcIA-specific antibody responses. Vaccination with a combination of PA- and BcIA-encoding pDNA led to significantly better survival than immunisation with only PA- or only BcIA-encoding plasmids. (c) 2005 Elsevier Ltd. All rights reserved.