DETERMINATION OF ANTI-THROMBIN ACTIVITY BY AN AMIDOLYTIC AND A CLOTTING PROCEDURE
DETERMINATION OF ANTI-THROMBIN ACTIVITY BY AN AMIDOLYTIC AND A CLOTTING PROCEDURE
复制标题
DOI：
10.1136/jcp.32.1.21
复制
发表时间：
1979-01-01
影响因子：
3.4
通讯作者：
CERQUETI, AR
中科院分区：
文献类型：
--
作者：
FRIGOLA, A;ANGELONI, S;CERQUETI, AR
使用酰胺分解法（底物Chromozym TH）和凝血法测定血浆抗凝血酶活性。在76名医院门诊患者中发现的平均抗凝血酶值为9.4 μ mol/min/ml，采用酰胺分解程序，抗凝血酶活性为100.1%，采用凝血程序。两种方法的相关性良好（r = 0.85，P < 0.01），重复性好。变异系数为5.9和8.8%，分别获得，与酰胺分解和凝血程序。在纤维蛋白原降解产物水平非常高的情况下，在凝血程序中观察到假性升高的抗凝血酶活性水平，但酰胺分解法基本上不受影响。这两种方法都适用于测定抗凝血酶活性，但一个良好的标准化酰胺分解程序具有优势。
Plasma antithrombin activity was measured using an amidolytic method (substrate Chromozym TH) and a clotting method. The mean antithrombin values found in 76 hospital outpatients were 9.4 .mu.mol/min per ml with the amidolytic procedure and 100.1% of antithrombin activity with the clotting procedure. The 2 methods correlated fairly well (r [correlatin coefficient] = 0.85, P < 0.01) and showed satisfactory reproducibility. Coefficients of variation of 5.9 and 8.8% were obtained, respectively, with the amidolytic and the clotting procedures. With very high levels of fibrinogen degradation products, falsely elevated antithrombin activity levels were observed wtih the clotting procedure but the amidolytic method is essentially unaffected. Both methods are suitable for determining antithrombin activity but a well-standardized amidolytic procedure has advantages.