Rapid size characterization of silver nanoparticles by single particle ICP-MS and isotope dilution
Rapid size characterization of silver nanoparticles by single particle ICP-MS and isotope dilution
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DOI：
10.1039/c4ja00115j
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发表时间：
2014-06
影响因子：
3.4
通讯作者：
L. Telgmann;C. Metcalfe;H. Hintelmann
中科院分区：
文献类型：
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作者：
L. Telgmann;C. Metcalfe;H. Hintelmann
银纳米颗粒(AgNP)在消费品中的应用日益广泛，其潜在的环境释放性要求对其毒性、稳定性和归宿进行深入的研究。需要能够在低浓度和复杂基质中检测和表征AgNPs的分析方法。单粒子电感耦合等离子体质谱(SPICPMS)是近年来出现的一种适用于低浓度纳米粒子定量和尺寸测定的可靠技术。然而，采用SPICPMS进行粒度测定依赖于纳米颗粒或元素标准溶液的外部校准。在这里，介绍了一种新的方法，使用内标法和同位素稀释来确定不同的AgNPs的大小。外部校准变得不必要，导致更快速的纳米颗粒表征和对基质效应的更强的稳健性。通过测定50 nm柠檬酸盐包被和80 nm PVP包被的109Ag富银标准银纳米粒的大小，验证了该方法的有效性。该方法重现性好，与已建立的方法结果吻合较好。用该方法成功测定了废水和河水中AgNPs的大小，证明了该方法即使在高基质含量的样品中也是可行的。样品制备只需要添加一个同位素浓缩标准，这使得当必须在许多不同的日子表征AgNPs时，该方法对于长期研究很有兴趣。例如，使用开发的同位素稀释内标法，在四天的时间内监测了不同条件下AgNPs的尺寸变化。
The increasing application of silver nanoparticles (AgNP) in consumer products and their potential release into the environment call for intensive investigation of their toxicity, stability, and fate. Analytical methods that are able to detect and characterize AgNPs at low concentrations and in complex matrices are needed. Single particle inductively coupled plasma mass spectrometry (spICP-MS) has in recent years emerged to a reliable technique suitable to quantify and size nanoparticles at low concentrations. However, the size determination by means of spICP-MS depends on external calibration with nanoparticle or element standard solutions. Here, a new approach is introduced using internal calibration with isotope dilution to determine the size of different AgNPs. External calibration becomes unnecessary, leading to a more rapid nanoparticle characterization and more robustness towards matrix effects. The power of the method is shown by determining the size of 50 nm citrate capped and 80 nm PVP capped AgNPs spiked with 109Ag enriched silver standard. The method is highly reproducible and shows good agreement with results obtained by established methods. The successful size determination of AgNPs in wastewater and river water using this method demonstrates its practicability even in samples with high matrix loads. Sample preparation only requires the addition of an isotope enriched standard, which makes the method interesting for long-term studies when AgNPs have to be characterized on many different days. As an example, the size alteration of AgNPs under different conditions was monitored over a period of four days, employing the developed method of internal calibration with isotope dilution.