Complete sequence and genetic characterization of a novel insect-specific reovirus discovered from Laodelphax striatellus
Complete sequence and genetic characterization of a novel insect-specific reovirus discovered from Laodelphax striatellus
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DOI：
10.1016/j.virol.2022.03.011
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发表时间：
2022-04-07
期刊：
影响因子：
3.7
通讯作者：
Li, Jun-Min
中科院分区：
文献类型：
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作者：
Lu, Gang;Zhang, Xiao-Di;Li, Jun-Min
褐飞虱Laodelphax striatellus(半翅目：飞蝇科)是几种重要植物病毒的有效媒介。在本研究中，在纹状体中发现了一种新的呼肠孤病毒样病毒，命名为灰飞虱呼肠孤病毒(Laodelphax striatellus rev，LSRV)。LSRV基因组全长28,207个核苷酸，由10个片段组成，编码11个推导蛋白。所有基因组片段在5&Prime；-末端的AGUAA和在3-Prime；-末端的GUUGUC和片段特有的反向末端重复序列中保守。此外，基因组特征和系统发育分析表明LSRV是斐济病毒属的一个新成员。重要的是，LSRV在各种组织中广泛分布，在成体头部表达最高。但LSRV不能在水稻植株上水平复制。此外，LSRV来源的小干扰RNA的典型图谱表明宿主抗病毒RNA干扰途径参与了LSRV的感染。综上所述，LSRV可能是呼肠孤病毒目FijiVirus属的一个新种。
The small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae), is an efficient vector of several economically important plant viruses. In this study, a novel reovirus-like virus was identified in L. striatellus, named Laodelphax striatellus reovirus (LSRV). The complete genome of LSRV was 28,207 nt, comprising of ten segments encoded 11 deduced proteins. All genome segments were conserved with AGUAA at the 5 & PRIME;-terminal and GUUGUC at 3 & PRIME;-terminal and segment-specific inverted terminal repeats. In addition, genomic characteristics and phylogenetic analysis suggested that LSRV was a new member of genus Fijivirus. Importantly, LSRV was widely distributed in various tissues and highest expressed in adult heads. However, LSRV was unable to horizontal replication in rice plants. Moreover, typical profiles of LSRV-derived small interfering RNAs indicated host antiviral RNA interference pathway was involved in LSRV infection. In conclusion, LSRV may be considered as a new species of the genus Fijivirus in the order Reovirales.