ZmbZIP4 Contributes to Stress Resistance in Maize by Regulating ABA Synthesis and Root Development
ZmbZIP4 Contributes to Stress Resistance in Maize by Regulating ABA Synthesis and Root Development
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ZmbZIP4 通过调节 ABA 合成和根部发育有助于玉米抗逆性
DOI：
10.1104/pp.18.00436
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发表时间：
2018-10-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Juren
中科院分区：
文献类型：
--
作者：
Ma, Haizhen;Liu, Can;Zhang, Juren
在植物中，bZIP（碱性亮氨酸拉链）转录因子调节多种过程，如发育和胁迫反应。然而，很少有这些转录因子的功能特点，在玉米（玉米）。在这项研究中，我们的特点是bZIP转录因子基因ZmbZIP4从玉米。ZmbZIP4在玉米不同器官中差异表达，并且在幼苗中受到高盐、干旱、高温、低温和脱落酸处理的诱导。酵母中的反式激活测定证明ZmbZIP4作为转录激活因子起作用。通过免疫沉淀测序对ZmbZIP4靶点的全基因组筛选显示，ZmbZIP4可以正向调节许多应激反应基因，如ZmLEA2，ZmRD20，ZmRD21，ZmRab18，ZmNHX 3，ZmGEA6和ZmERD，以及一些脱落酸合成相关基因，包括NCED，ABAI，AAO 3和LOSS。此外，ZmbZIP4靶向一些根发育相关基因，包括ZmLRP 1、ZmSCR、ZmIAAS、ZmIAA14、ZmARF 2和ZmARF 3，并且ZmbZIP4的过表达导致侧根数量增加、主根更长和根系改善。通过ZmbZIP4的过表达增加脱落酸合成也可以增加植物抵抗非生物胁迫的能力。因此，ZmbZIP4是植物非生物胁迫响应的正调节剂，并且参与玉米的根发育。
In plants, bZIP (basic leucine zipper) transcription factors regulate diverse processes such as development and stress responses. However, few of these transcription factors have been functionally characterized in maize (Zea mays). In this study, we characterized the bZIP transcription factor gene ZmbZIP4 from maize. ZmbZIP4 was differentially expressed in various organs of maize and was induced by high salinity, drought, heat, cold, and abscisic acid treatment in seedlings. A transactivation assay in yeast demonstrated that ZmbZIP4 functioned as a transcriptional activator. A genome-wide screen for ZmbZIP4 targets by immunoprecipitation sequencing revealed that ZmbZIP4 could positively regulate a number of stress response genes, such as ZmLEA2, ZmRD20, ZmRD21, ZmRab18, ZmNHX3, ZmGEA6, and ZmERD, and some abscisic acid synthesis-related genes, including NCED, ABAI, AAO3, and LOSS. in addition, ZmbZIP4 targets some root development-related genes, including ZmLRP1, ZmSCR, ZmIAAS, ZmIAA14, ZmARF2, and ZmARF3, and overexpression of ZmbZIP4 resulted in an increased number of lateral roots, longer primary roots, and an improved root system. Increased abscisic acid synthesis by overexpression of ZmbZIP4 also can increase the plant's ability to resist abiotic stress. Thus, ZmbZIP4 is a positive regulator of plant abiotic stress responses and is involved in root development in maize.