Bacterial mode of replication with eukaryotic-like machinery in a hyperthermophilic archaeon
Bacterial mode of replication with eukaryotic-like machinery in a hyperthermophilic archaeon
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DOI：
10.1126/science.288.5474.2212
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发表时间：
2000-06-23
期刊：
影响因子：
56.9
通讯作者：
Forterre, P
中科院分区：
文献类型：
--
作者：
Myllykallio, H;Lopez, P;Forterre, P
尽管现有的古细菌序列信息量迅速增加，但对生命第三领域中遗传物质的复制知之甚少。我们确定了一个单一的起点，双向复制的Pyrococcus abyssi通过计算机分析的累积寡聚体偏斜和早期复制的染色体片段的鉴定。在3种Pyrococcus中，复制起点高度保守，其周围聚集了多个真核生物的复制基因，与细菌一样，含有复制终点的染色体区域也是基因组改组的热点。因此，尽管细菌和古细菌的复制蛋白有很大的不同，但它们在原核生物中以相似的方式复制染色体。
Despite a rapid increase in the amount of available archaeal sequence information, little is known about the duplication of genetic material in the third domain of life. We identified a single origin of bidirectional replication in Pyrococcus abyssi by means of in silico analyses of cumulative oligomer skew and the identification of an early replicating chromosomal segment. The replication origin in three Pyrococcus species was found to be highly conserved, and several eukaryotic-like DNA replication genes were clustered around it. As in Bacteria, the chromosomal region containing the replication terminus was a hot spot of genome shuffling. Thus, although bacterial and archaeal replication proteins differ profoundly, they are used to replicate chromosomes in a similar manner in both prokaryotic domains.