NR2B-containing N-methyl-D-aspartate subtype glutamate receptors regulate the acute stress effect on hippocampal long-term potentiation/long-term depression in vivo
NR2B-containing N-methyl-D-aspartate subtype glutamate receptors regulate the acute stress effect on hippocampal long-term potentiation/long-term depression in vivo
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DOI：
10.1097/01.wnr.0000227994.07799.6c
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发表时间：
2006-08
期刊：
影响因子：
1.7
通讯作者：
Meina Wang;Ya Yang;Zhifang Dong;Jun Cao;Lin Xu
中科院分区：
文献类型：
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作者：
Meina Wang;Ya Yang;Zhifang Dong;Jun Cao;Lin Xu
行为压力会促进长期抑郁，但会损害海马体的长期增强。最近的体外证据表明，含有 NR2B 的 N-甲基-D-天冬氨酸亚型谷氨酸受体拮抗剂 Ro25-6981 可预防行为压力促进的海马长期抑郁。然而，Ro25-6981是否影响应激条件下体内海马长期抑制和长期增强诱导尚不清楚。在这里，我们发现在低频刺激前30分钟输注Ro25-6981（6μl中2.3μg，脑室内10分钟）可以防止麻醉成年大鼠急性应激引起的海马长期抑郁。此外，在高频刺激前30分钟输注Ro25-6981可逆转应激损伤的海马长时程增强。这些结果表明，含有NR2B的N-甲基-D-天冬氨酸亚型谷氨酸受体对于行为应激对海马长期抑郁和体内长期增强的影响至关重要。
Behavioral stress facilitates long-term depression but impairs long-term potentiation in the hippocampus. Recent evidence in vitro demonstrates that the NR2B-containing N-methyl-D-aspartate subtype glutamate receptor antagonist Ro25-6981 prevents the behavioral stress-facilitated hippocampal long-term depression. It is, however, unknown whether Ro25-6981 influences hippocampal long-term depression and long-term potentiation induction in vivo under stressful condition. Here, we found that infusion of Ro25-6981 (2.3 μg in 6 μl, intracerebroventricular for 10 min) 30 min before low-frequency stimulation prevented the facilitation of hippocampal long-term depression by acute stress in anesthetized adult rats. Moreover, infusion of Ro25-6981 30 min before high-frequency stimulation reversed stress-impaired hippocampal long-term potentiation. These results suggest that the NR2B-containing N-methyl-D-aspartate subtype glutamate receptors are crucial for the effects of behavioral stress on hippocampal long-term depression and long-term potentiation in vivo.