Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease.
Increased glucose transporter-1 expression on intermediate monocytes from HIV-infected women with subclinical cardiovascular disease.
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DOI：
10.1097/qad.0000000000001320
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发表时间：
2017-01-14
期刊：
影响因子：
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通讯作者：
Anzinger JJ
中科院分区：
文献类型：
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作者：
Butterfield TR;Hanna DB;Kaplan RC;Kizer JR;Durkin HG;Young MA;Nowicki MJ;Tien PC;Golub ET;Floris-Moore MA;Titanji K;Fischl MA;Heath SL;Martinson J;Crowe SM;Palmer CS;Landay AL;Anzinger JJ
艾滋病毒感染者（PLWH）有慢性免疫激活和心血管疾病（CVD）风险增加。单核细胞和T淋巴细胞的活化导致葡萄糖转运蛋白-1（GLUT 1）的上调以实现有效功能。PLWH具有增加的表达GLUT 1的单核细胞和T淋巴细胞的百分比，但尚不清楚这些细胞是否与CVD相关。我们评估了GLUT 1和CD 38在HIV感染的亚临床CVD妇女的单核细胞和T淋巴细胞群上的表达。从妇女跨部门HIV研究中确定了右颈总动脉和分叉处年龄调整的内膜中层厚度（IMT）>第75百分位数（n = 15）和<第25百分位数（n=15）的参与者。各组按年龄、人种/种族、吸烟状况和CD 4计数进行匹配。除了一名高IMT和一名低IMT参与者外，所有妇女都接受了抑制性抗逆转录病毒治疗。使用流式细胞术评价单核细胞和T淋巴细胞群体的GLUT 1和CD 38表达。与低IMT女性相比，来自高IMT女性的中等单核细胞的GLUT 1（310 MFI vs. 210 MFI，p=0.024）（66.4% vs. 48.5%，p=0.031）和CD 38（339 MFI vs. 211 MFI，p=0.002）（10.5% vs. 3.8%，p=0.0002）表达显著增加。高和低IMT参与者在经典单核细胞、非经典单核细胞、CD 4+和CD 8 + T淋巴细胞上的GLUT 1或CD 38表达没有差异。HIV感染的亚临床CVD妇女中GLUT 1表达的中间单核细胞升高这些细胞可能有助于PLWH中CVD的发展，并可能成为限制炎症的新靶点。
People living with HIV (PLWH) have chronic immune activation and increased cardiovascular disease (CVD) risk. Activation of monocytes and T lymphocytes causes up-regulation of glucose transporter-1 (GLUT1) for efficient function. PLWH have an increased percentage of GLUT1-expressing monocytes and T lymphocytes, but it is unclear if these cells are associated with CVD. We evaluated expression of GLUT1 and CD38 on monocyte and T lymphocyte populations from HIV-infected women with subclinical CVD. Participants with >75th percentile (n=15) and <25th percentile (n=15) age-adjusted intima-media thickness (IMT) at the right common carotid artery and bifurcation were identified from the Women's Interagency HIV Study. Groups were matched by age, race/ethnicity, smoking status, and CD4 count. All women were receiving suppressive antiretroviral therapy except for one high and one low IMT participant. Monocyte and T lymphocyte populations were evaluated for GLUT1 and CD38 expression using flow cytometry. Intermediate monocytes from high IMT women had significantly increased expression of GLUT1 (310 MFI vs. 210 MFI, p=0.024) (66.4% vs. 48.5%, p=0.031) and CD38 (339 MFI vs. 211 MFI, p=0.002) (10.5% vs. 3.8%, p=0.0002) compared to women with low IMT. High and low IMT participants showed no differences in GLUT1 or CD38 expression on classical monocytes, non-classical monocytes, CD4+ and CD8+ T lymphocytes. GLUT1-expressing intermediate monocytes are elevated in HIV-infected women with subclinical CVD. These cells may contribute to development of CVD in PLWH and could be a novel target to limit inflammation.