THE PORA GENE IN SEROGROUP-A MENINGOCOCCI - EVOLUTIONARY STABILITY AND MECHANISM OF GENETIC-VARIATION
THE PORA GENE IN SEROGROUP-A MENINGOCOCCI - EVOLUTIONARY STABILITY AND MECHANISM OF GENETIC-VARIATION
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DOI：
10.1111/j.1365-2958.1994.tb01014.x
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发表时间：
1994-04-01
影响因子：
3.6
通讯作者：
MAIDEN, MCJ
中科院分区：
文献类型：
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作者：
SUKER, J;FEAVERS, IM;MAIDEN, MCJ
对来自 55 种 A 血清群脑膜炎奈瑟菌菌株的血清亚型分型抗原（1 类外膜蛋白 (PorA)）编码变体的基因进行了分子分析。这些基因在进化上是稳定的，并且表现出有限范围的遗传变异，主要是通过重组产生的。基因序列的翻译揭示了蛋白质可变区中总共 19 个不同的氨基酸序列，其中 6 个氨基酸序列不能被目前可用的血清亚型分型单克隆抗体识别。了解这些氨基酸序列可以合理地重新分配血清亚型名称。将完整基因与血清群 B 和 C 脑膜炎球菌的 porA 基因序列进行比较表明，血清群 A 拥有来自全球分布基因库的有限数量的可能的 porA 基因。每个血清群 A 亚群的特征是四种 porA 基因类型之一，可能是在亚群分化时获得的，该基因类型在数十年和流行病学传播过程中保持稳定。与其他可变基因（pil 和 iga）的比较表明，这三个等位基因在亚组内独立分类，表明它们的基因类型比它们出现的亚组更古老。
Molecular analyses were applied to the genes encoding variants of the serosubtyping antigen, the class 1 outer membrane protein (PorA), from 55 serogroup A Neisseria meningitidis strains. These genes were evolutionarily stable and exhibited a limited range of genetic variation, primarily generated by recombination. Translation of the gene sequences revealed a total of 19 distinct amino acid sequences in the variable regions of the protein, 6 of which were not recognized by currently available serosubtyping monoclonal antibodies. Knowledge of these amino acid sequences permitted a rational re-assignment of serosubtype names. Comparison of the complete genes with porA gene sequences from serogroup B and C meningococci showed that serogroup A possessed a limited number of the possible porA genes from a globally distributed gene pool. Each serogroup A subgroup was characterized by one of four porA gene types, probably acquired upon subgroup divergence, which was stable over periods of decades and during epidemiological spread. Comparison with other variable genes (pil and iga) indicated that the three alleles were independently assorted within the subgroup, suggesting that their gene types were older than the subgroups in which they occurred.