A genetic linkage map of the diplosporous chromosomal region in Taraxacum officinale (common dandelion; Asteraceae)
A genetic linkage map of the diplosporous chromosomal region in Taraxacum officinale (common dandelion; Asteraceae)
复制标题
蒲公英（普通蒲公英；菊科）双孢子染色体区域的遗传连锁图
DOI：
--
复制
发表时间：
2004
影响因子：
5.4
通讯作者：
P. J. V. Dijk
中科院分区：
文献类型：
--
作者：
K. Vijverberg;R. Hulst;P. Lindhout;P. J. V. Dijk
本研究利用扩增片段长度多态性技术（AFLP）对蒲公英（Taraxacum officinale） 73个分离群体的双孢子染色体区域进行了定位。蒲公英为研究无融合生殖的遗传学、生态学和进化提供了一个模型系统。本属包括有性二倍体和无融合多倍体，大部分是三倍体植物。无生殖蒲公英是双孢子，孤雌生殖，并有自主胚乳形成。先前的研究表明，这三种无融合基因在蒲安英中受多个基因座控制，双孢子遗传是一种显性的单基因性状（Ddd； DIP）。大量分离分析提供了34个与DIP相关的AFLP标记，并与两个微卫星标记一起用于定位该性状。图谱长度为18.6 cM， DIP两侧均有标记，分别对应5.9 cM和12.7 cM。没有一个标记与DIP完全共分离。选择8个标记进行pcr标记开发，其中2个成功转化。与迄今为止所有其他无减数分裂定位研究相反，我们的结果显示没有证据表明蒲公英DIP位点周围的重组受到抑制。未发现DIP与非DIP同源位点序列分化的明显证据，DIP位点未发现半合性。这些结果可能表明蒲公英中无融合现象的发生相对较晚。
In this study, we mapped the diplosporous chromosomal region in Taraxacum officinale, by using amplified fragment length polymorphism technology (AFLP) in 73 plants from a segregating population. Taraxacum serves as a model system to investigate the genetics, ecology, and evolution of apomixis. The genus includes sexual diploid as well as apomictic polyploid, mostly triploid, plants. Apomictic Taraxacum is diplosporous, parthenogenetic, and has autonomous endosperm formation. Previous studies have indicated that these three apomixis elements are controlled by more than one locus in Taraxacum and that diplospory inherits as a dominant, monogenic trait (Ddd; DIP). A bulked segregant analysis provided 34 AFLP markers that were linked to DIP and were, together with two microsatellite markers, used for mapping the trait. The map length was 18.6 cM and markers were found on both sides of DIP, corresponding to 5.9 and 12.7 cM, respectively. None of the markers completely co-segregated with DIP. Eight markers were selected for PCR-based marker development, of which two were successfully converted. In contrast to all other mapping studies of apomeiosis to date, our results showed no evidence for suppression of recombination around the DIP locus in Taraxacum. No obvious evidence for sequence divergence between the DIP and non-DIP homologous loci was found, and no hemizygosity at the DIP locus was detected. These results may indicate that apomixis is relatively recent in Taraxacum.
无性真核生物中的杂合性、异形性和系统发育树。
影响因子：
3.3
作者：
BirkyJr,CW
通讯作者：
BirkyJr,CW