INFLAMMATORY CELLS AND THE EPITHELIUM - MAST-CELL NERVE INTERACTIONS IN THE LUNG INVITRO AND INVIVO
INFLAMMATORY CELLS AND THE EPITHELIUM - MAST-CELL NERVE INTERACTIONS IN THE LUNG INVITRO AND INVIVO
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DOI：
10.1164/ajrccm/138.6_pt_2.s31
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发表时间：
1988-12-01
期刊：
影响因子：
--
通讯作者：
MARSHALL, J
中科院分区：
文献类型：
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作者：
BIENENSTOCK, J;PERDUE, M;MARSHALL, J
摘要提出了一种假说，即呼吸道上皮内及下方的肥大细胞与神经有功能联系，形成一个内环境平衡调节单位。在炎症过程中，肥大细胞既可以在原位产生，也可以通过渗透产生，因为上皮含有肥大细胞前体，并在体外产生支持其生长的因子。结构研究表明，肥大细胞与肺中的神经相联系。利用组织培养模型，我们发现交感神经与大鼠嗜碱粒细胞白血病(RBL)细胞形成了持久的接触。电生理研究表明，神经接触使RBL膜电导增加，外源性P物质(SP)可模拟这种作用。Ussing小室致敏大鼠气管粘膜实验显示肥大细胞与SP能神经相互作用的功能证据：由抗原引起的短路电流变化可被肥大细胞稳定剂多沙曲唑阻断，并可被苏打！用辣椒素对新生儿进行预处理。体内实验表明，致敏大鼠经抗原攻击后，99MTC_DTPA气雾剂的肺清除率增加。这被新生儿辣椒素治疗所阻断，再次牵涉到含有SP的神经。
SUMMARY A hypothesis is presented that mast cells in and below the epithelium of the respiratory tract show functional association with nerves to form a homeostatic regulatory unit. During inflammation, mast cells may arise in situ as well as by infiltration because epithelium contains both mast cell precursors and produces factors that support their growth in vitro. Structural studies show that mast cells associate with nerves in the lung. Using a tissue culture model, we showed that sympathetic nerves formed lasting contacts with rat basophilic leukemia (RBL) cells. Electrophysiologic studies showed that nerve contact increases RBL membrane conductance, which can be mimicked by exogenous substance P (SP). Experiments with sensitized rat tracheal mucosa in Ussing chambers showed functional evidence of interaction of mast cells with SP-containing nerves: changes in short circuit current caused by antigen were blocked by the mast cell stabilizer doxantrazole and reduced by SODA! by neonatal pretreatment with capsaicin. Experiments in vivo showed that lung clearance of the aerosol probe 99 mTc_DTPA was increased by antigen challenge in sensitized rats. This was blocked by neonatal capsaicin treatment, again implicating SP-containing nerves.