COMPARATIVE BIOLOGICAL RESPONSES OF RABBITS INFECTED WITH HUMAN T-LYMPHOTROPIC VIRUS TYPE-I ISOLATES FROM PATIENTS WITH LYMPHOPROLIFERATIVE AND NEURODEGENERATIVE DISEASE
COMPARATIVE BIOLOGICAL RESPONSES OF RABBITS INFECTED WITH HUMAN T-LYMPHOTROPIC VIRUS TYPE-I ISOLATES FROM PATIENTS WITH LYMPHOPROLIFERATIVE AND NEURODEGENERATIVE DISEASE
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DOI：
10.1002/ijc.2910500125
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发表时间：
1992-01-02
影响因子：
6.4
通讯作者：
COCKERELL, GL
中科院分区：
文献类型：
--
作者：
LAIRMORE, MD;ROBERTS, B;COCKERELL, GL
实验兔模型用于确定宿主对各种人嗜T淋巴细胞病毒I型（HTLV-I）毒株感染的反应。用来自成人T细胞白血病/淋巴瘤患者或HTLV-I相关性脊髓病患者的经致死性辐照的HTLV-I感染细胞系接种7组家兔，每组4 - 5只。4个单独的对照组，每组2只家兔，接种相似制备的来自家兔或人的HTLV-I阴性细胞。通过免疫印迹法评估抗病毒抗体应答，并使用自动细胞计数器和细胞学染色测量血液学参数。通过共培养和HTLV-I抗原捕获试验以及通过聚合酶链反应（PCR）扩增外周血单个核细胞（PBMC）或组织中的HTLV-I DNA来确定攻毒兔的病毒学状态。HTLV-I接种物可根据其对家兔的感染性分为几组：高度感染性菌株引起强烈的血清学反应，并经常通过抗原和PCR检测在组织中检出，而其他菌株感染性中等至较差，诱导弱抗体反应，很少通过抗原和PCR检测检出。总体而言，PBMC似乎具有最大量的含HTLV-I的细胞，而骨髓是较差的病毒来源。在24周的感染过程中，没有明显的临床或血液学异常。两者合计，我们的研究结果表明，有异质性的生物反应HTLV-I感染，这是，在一定程度上，依赖于感染病毒株。
An experimental rabbit model was used to determine host responses to infection by various human T-lymphotropic virus type-I (HTLV-I) strains. Seven groups of 4 to 5 rabbits each were inoculated with lethally-irradiated HTLV-I-infected cell lines derived from patients with adult T-cell leukemia\lymphoma or from patients with HTLV-I-associated myelopathy. Four separate control groups of 2 rabbits each were inoculated with similarly prepared HTLV-I-negative cells derived from rabbits or humans. Anti-viral antibody responses were assessed by immunoblot assay and hematologic parameters were measured using automated cell counters and cytologic staining. The virologic status of challenged rabbits was determined by co-culture and HTLV-I antigen capture assay, as well as by polymerase chain reaction (PCR) amplification of HTLV-I DNA from peripheral blood mononuclear cells (PBMC) or tissues. The HTLV-I inocula could be separated into groups based upon their infectivity to rabbits: highly infectious strains elicited intense serologic responses and were detected frequently in tissues by antigen and PCR assays, while other strains were moderately to poorly infectious, induced weak antibody responses and were infrequently detected by antigen and PCR assays. Overall, PBMC appeared to have the greatest quantity of HTLV-I containing cells, while bone marrow was a poor source of virus. No clinical or hematologic abnormalities were evident during the 24-week course of infection. Taken together, our results suggest there is heterogeneity in the biological response to HTLV-I infection which is, in part, dependent on the infecting strain of virus.