Neural progenitor cells transplanted into the uninjured brain undergo targeted migration after stroke onset
Neural progenitor cells transplanted into the uninjured brain undergo targeted migration after stroke onset
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DOI：
10.1002/jnr.21542
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发表时间：
2008-03-01
影响因子：
4.2
通讯作者：
Steinberg, Gary
中科院分区：
文献类型：
--
作者：
Guzman, Raphael;Bliss, Tonya;Steinberg, Gary
内源性神经干细胞通常驻留在未受伤的啮齿动物大脑中的小生境（室下区）中。中风后，这些细胞变得更加增殖并从室下区迁移到周围的实质。目前尚不清楚这种中风诱发的行为是否是由于生态位的变化或在梗死区引入有吸引力的线索，或两者兼而有之。一个相关的问题是移植的神经干细胞如何应对随后的损伤，包括外源干细胞是否具有可塑性以应对植入后随后的损伤。我们通过将神经祖细胞（NPC）移植到未受伤的大脑中，然后让动物遭受中风来解决这个问题。通过用超顺磁性氧化铁颗粒标记移植的 NPC，并在植入期间和中风后通过高分辨率磁共振成像 (MRI) 对它们进行成像，我们能够在体内跟踪移植的 NPC。我们发现，潜伏的移植 NPC 可以响应中风而被激活，并表现出定向迁移到实质中，类似于内源性神经 NPC，无需利基环境。 (c) 2007 年 Wiley-Liss, Inc.
Endogenous neural stem cells normally reside in their niche, the subventricular zone, in the uninjured rodent brain. Upon stroke, these cells become more proliferative and migrate away from the subventricular zone into the surrounding parenchyma. It is not known whether this stroke-induced behavior is due to changes in the niche or introduction of attractive cues in the infarct zone, or both. A related question is how transplanted neural stem cells respond to subsequent insults, including whether exogenous stem cells have the plasticity to respond to subsequent injuries after engraftment. We addressed this issue by transplanting neural progenitor cells (NPCs) into the uninjured brain and then subjecting the animal to stroke. We were able to follow the transplanted NPCs in vivo by labeling them with superparamagnetic iron oxide particles and imaging them via high-resolution magnetic resonance imaging (MRI) during engraftment and subsequent to stroke. We find that transplanted NPCs that are latent can be activated in response to stroke and exhibit directional migration into the parenchyma, similar to endogenous neural NPCs, without a niche environment. (c) 2007 Wiley-Liss, Inc.