Control of Cell Surface and Functions by Layer-by-Layer Nanofilms
Control of Cell Surface and Functions by Layer-by-Layer Nanofilms
复制标题
DOI：
10.1021/la903738n
复制
发表时间：
2010-04-20
期刊：
影响因子：
3.9
通讯作者：
Akashi, Mitsuru
中科院分区：
文献类型：
--
作者：
Kadowaki, Koji;Matsusaki, Michiya;Akashi, Mitsuru
通过层层（LbL）组装技术直接在小鼠 L929 成纤维细胞表面制备各种纳米尺寸的多层膜，以控制细胞表面微环境和细胞功能，如活力、形态和增殖。 LbL 纳米膜的种类强烈影响细胞的形态和生长。聚电解质（PE）多层诱导粘附细胞的圆形形态，尽管多层的每个成分都具有高细胞相容性，而具有EN结合域相互作用的纤连蛋白（FN）-明胶（G）和硫酸葡聚糖（DS）多层（EN膜）显示出与对照细胞（无膜）相似的旧细胞的扩展形态。 PE 和 EN 膜的细胞增殖存在明显差异。表面具有 EN 膜的细胞显示出良好的增殖曲线，与膜厚度无关，但使用 PE 膜时未观察到细胞增殖，尽管细胞在培养期间存活。荧光显微镜和扫描电子显微镜观察清楚地表明，孵育 24 小时后，EN 膜在细胞表面呈现纳米级网状形态，而 PE 膜在细胞表面呈现均匀的膜形态。这些纳米网形态似乎与天然细胞外基质的纤维结构相似。这项研究的结果表明，直接在细胞表面制备的LbL纳米膜的成分、电荷和形态强烈影响细胞功能，并且这些LbL纳米膜对细胞功能的影响与在基底上制备的PE膜有很大不同。在细胞表面制备 LbL 纳米膜可能是控制细胞功能的一种新颖且有趣的技术。
Various nanometer-sized multilayers were directly prepared onto the surface a mouse L929 fibroblast cells by a layer-by-layer (LbL) assembly technique to control the cell surface microenvironment and cell functions, such as viability, morphology, and proliferation. The species of LbL nanofilms strongly affected the cell morphology and growth. Polyelectrolyte (PE) multilayers induced a round-shaped morphology of the adhered cells, although each component of the multilayers had high cytocompatibility, whereas fibronectin (FN)-gelatin (G) and -dextran sulfate (DS) multilayers with EN-binding domain interactions (EN films) showed extended morphologies oldie cells similar to that of control cells (without films). A clear difference in cell proliferation was observed for PE and EN films. The cells with EN films on their surfaces showed good proliferation profiles independent of the film thickness, hut cell proliferation was not observed using the PE films although the cells survived during the culture period. Fluorescence microscopic and scanning electron microscopic observations clearly suggested a nanometer-sized meshwork morphology of the EN films on the cell surface after 24 h of incubation, whereas the PE films showed homogeneous film morphologies on the cell surface. These nanomeshwork morphologies seemed to be similar to the fibrous structure of natural extracellular matrix. The results of this study demonstrated that the components, charge, and morphology of LbL nanofilms prepared directly on the cell surface strongly affected cell functions, and the effects of these LbL nanofilms on cell functions differed vastly as compared to PE films prepared on a substrate. The preparation of LbL nanofilms onto a cell surface might be a novel and interesting technique to control cell functions.