Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements
Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements
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DOI：
10.1002/pmic.201500118
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发表时间：
2015-12-01
期刊：
影响因子：
3.4
通讯作者：
Cristobal, Susana
中科院分区：
文献类型：
--
作者：
Campos, Alexandre;Apraiz, Itxaso;Cristobal, Susana
海洋贻贝的先天免疫提供了对病原体入侵和炎症的保护。在这方面，贻贝血淋巴在动物先天反应中起主要作用。尽管这种体液在确定动物的生理状况方面很重要，但对细胞和体液反应的分子机制知之甚少。在这项工作中，我们已经应用了MS（纳米-LC-MS/MS）策略整合基因组和转录组数据，目的是：（i）确定主要的蛋白质功能组的特点血淋巴和（ii）映射的天然免疫的元素在海洋贻贝紫贻贝血淋巴蛋白质组。在样品分析和基于MS/MS数据比较的第一次蛋白质鉴定之后，使用公共数据库（nrNCBI）信息用blast注释具有未知功能的蛋白质。总共鉴定了595种血淋巴蛋白，具有高置信度并进行了注释。这些蛋白质包括主要的细胞代谢过程：能量产生和生物分子的代谢，以及与氧化应激防御，异生物质解毒，药物代谢和免疫反应相关的过程。在M.美味。数据可通过ProteomeXchange获得，标识符为PXD 001951（http：//proteomecentral.proteomexchange.org/soft/PXD 001951）。
The marine mussel innate immunity provides protection to pathogen invasion and inflammation. In this regard, the mussel hemolymph takes a main role in the animal innate response. Despite the importance of this body fluid in determining the physiological condition of the animal, little is known about the molecular mechanisms underlying the cellular and humoral responses. In this work, we have applied a MS (nano-LC-MS/MS) strategy integrating genomic and transcriptomic data with the aim to: (i) identify the main protein functional groups that characterize hemolymph and (ii) to map the elements of innate immunity in the marine mussel Mytilus edulis hemolymph proteome. After sample analysis and first protein identification based on MS/MS data comparison, proteins with unknown functions were annotated with blast using public database (nrNCBI) information. Overall 595 hemolymph proteins were identified with high confidence and annotated. These proteins encompass primary cellular metabolic processes: energy production and metabolism of biomolecules, as well as processes related to oxidative stress defence, xenobiotic detoxification, drug metabolism, and immune response. A group of proteins was identified with putative immune effector, receptor, and signaling functions in M. edulis. Data are available via ProteomeXchange with identifier PXD001951 (http://proteomecentral.proteomexchange.org/dataset/PXD001951).