Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories
Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories
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DOI：
10.1101/2023.01.04.522730
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发表时间：
2023-01
期刊：
影响因子：
13.6
通讯作者：
L. Piovani;Daniel J. Leite;L. A. Yañez Guerra;Fraser Simpson;J. Musser;Irepan Salvador-Martínez;
中科院分区：
文献类型：
--
作者：
L. Piovani;Daniel J. Leite;L. A. Yañez Guerra;Fraser Simpson;J. Musser;Irepan Salvador-Martínez;
浮游幼虫阶段在动物中广泛存在，但尚不清楚幼虫是否存在于动物的最后共同祖先中，或者他们是否由于共同的选择压力而多次进化。许多海洋幼虫至少在表面上是相似的，它们很小，通过跳动纤毛带游泳，并通过顶端器官结构感知环境。为了了解这些相似性，我们为两个动物门的海洋幼虫制作了单细胞图谱，并比较了它们的细胞类型。我们在两种幼虫的纤毛带细胞和顶器神经元之间发现了明显的相似之处，表明这些结构可能是同源的，这表明在这里分析的分支(Lophotrochozoa)内的幼虫是单一来源的。我们还在每个幼虫中发现了几个分支特有的创新，包括牡蛎幼虫中不同的肌细胞和壳腺细胞。牡蛎壳腺细胞表达许多新的基因，这使得以前对担轮动物幼虫的基因年龄估计过于年轻。
Pelagic larval stages are widespread across animals, yet it is unclear if larvae were present in the last common ancestor of animals or whether they evolved multiple times due to common selective pressures. Many marine larvae are at least superficially similar, they are small, swim through beating of ciliated bands and sense the environment with an apical organ structure. To understand these similarities, we have generated single cell atlases for marine larvae from two animal phyla and have compared their cell types. We found clear similarities among ciliary band cells and neurons of the apical organ in the two larvae pointing to possible homology of these structures suggesting a single origin of larvae within the clade analysed here (Lophotrochozoa). We also find several clade specific innovations in each larva, including distinct myocytes and shell gland cells in the oyster larva. Oyster shell gland cells express many novel genes which have made previous gene age estimates for trochophore larvae too young.