In Utero Stem Cell Transplantation: Potential Therapeutic Application for Muscle Diseases.
In Utero Stem Cell Transplantation: Potential Therapeutic Application for Muscle Diseases.
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DOI：
10.1155/2017/3027520
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发表时间：
2017
影响因子：
4.3
通讯作者：
Asakura A
中科院分区：
文献类型：
--
作者：
Chowdhury N;Asakura A
肌营养不良、肌病和创伤性肌肉损伤和丧失包括目前无法治愈的一大组病症。几十年来，成肌细胞移植一直被研究为治疗这些疾病的潜在方法。然而，目前的技术缺乏产生产生任何治疗益处所需的细胞数量的能力。子宫内干细胞移植到胚胎中已经研究了多年，主要是在造血细胞的背景下，并已显示出具有实验优势和治疗应用。此外，患者来源的细胞可用于通过子宫内注射实验性移植到非人动物胚胎中，因为在这种早期发育阶段不存在免疫应答。因此，我们提出子宫内移植作为一种潜在的方法，以产生患者来源的人源化骨骼肌以及肌肉干细胞在动物中用于治疗目的以及患者特异性药物筛选。
Muscular dystrophies, myopathies, and traumatic muscle injury and loss encompass a large group of conditions that currently have no cure. Myoblast transplantations have been investigated as potential cures for these conditions for decades. However, current techniques lack the ability to generate cell numbers required to produce any therapeutic benefit. In utero stem cell transplantation into embryos has been studied for many years mainly in the context of hematopoietic cells and has shown to have experimental advantages and therapeutic applications. Moreover, patient-derived cells can be used for experimental transplantation into nonhuman animal embryos via in utero injection as the immune response is absent at such early stages of development. We therefore propose in utero transplantation as a potential method to generate patient-derived humanized skeletal muscle as well as muscle stem cells in animals for therapeutic purposes as well as patient-specific drug screening.